摘要
目的:优化脑蛋白水解物的提取工艺。方法:以p H约为9的碱性提取液进行提取;以总氮含量、与脑蛋白水解物(丽珠赛乐)肽图的相似度的综合评分为指标,通过L9(34)正交试验优化猪脑(1 kg)水解工艺中胰蛋白酶的加入量、胃蛋白酶的加入量及水解时间,并进行工艺验证试验。结果:最优提取工艺为胰蛋白酶加入量8 g、胃蛋白酶加入量5 g、水解时间12 h;验证试验中所制脑蛋白水解物的总氮含量分别为5.20、5.18、5.20 mg/ml(RSD=2.22%,n=3),肽图相似度分别为93%、94%、96%(RSD=1.62%,n=3),综合评分分别为0.911、0.917、0.932(RSD=1.18%,n=3)。结论:优化的生产工艺合理、提取效率高,产物肽图相似度高。
OBJECTIVE： To optimize the extraction technology of cerebroprotein hydrolyzate. METHODS： It was extracted by alkaline extraction solution with pH approximately 9 ; using the comprehensive score of similarities of total nitrogen content and peptide map of cerebroprotein hydrolyzate （cerebrolisin） as index, L9 （3^4） orthogonal test was conducted to optimize the addition amounts of trypsin and pepsin, and hydrolysis time in pig brain （1 kg） hydrolysis technology, and the optimized technology was verified. RESULTS： Optimized extraction technology was as follows as trypsin adding 8 g, pepsin adding 5 g, hydrolysis time 12 h; the total nitrogen contents of obtained cerebroprotein hydrolyzate were 5.20, 5.18, 5.20 mg/ml （RSD=2.22%, n=3）, peptide figure similarities were 93%, 94%, 96% （RSD=1.62%,n=3）, comprehensive scores were 0.911, 0.917, 0.932 （RSD=1.18%, n = 3）, respectively. CONCLUSIONS： The optimized technology is rational with high extraction rate and peptide map similarity.
出处
《中国药房》
CAS
北大核心
2016年第28期3985-3987,共3页
China Pharmacy
关键词
脑蛋白水解物
提取工艺
正交试验
总氮含量
肽图相似度
Cerebroprotein hydrolyzate
Extraction technology
Orthogonal test
Total nitrogen content
Peptide map similarity
