摘要
目的观察癫痫患者咀清中高迁移率族蛋白B1（HMGB1）和Toll样受体4（TLR4）水平变化情况并探讨其临床意义。方法选择自2011年1月1日至2014年12月30日郑州大学第五附属医院神经内、外科收治的癫痫患者65例为癫痫组，同期体检的20例健康者为正常组。应用双抗体夹心酶联免疫吸附实验（ELASA）测定2组患者血清中HMGBl及TLR4水平，分析癫痫患者血清HMGB1及TLR4水平与药物治疗有效程度、癫痫发作平均时间、癫痫发作频率、癫痫病程、癫痫发作类型的关系，同时分析血清TLR4水平与HMGB1水平之间的相关性。结果癫痫组患者血清中HMGB1及TLR4水平均高于正常组：药物难治癫痫患者血清中HMGB1及TLR4水平均高于药物有效癫痫患者；癫痫发作平均时间〉5min患者血清中HMGB1及TLR4水平均高于发作平均时间≤5min患者；癫痫发作频率〉3次／月的患者血清中HMGB1及TLR4水平均高于癫痫发作频率≤3次／月患者；癫痫病程〉2年的患者血清中HMGB1及TLR4水平均高于癫痫病程≤2年的患者：上述指标差异均有统计学意义（P〈0．05）。部分性发作与全身性发作癫痫患者血清中HMGB1、TLR4水平差异无统计学意义（P〉0．05）。癫痫患者血清中TLR4与HMGBl呈正相关（r=0．926．P=-0．000）。结论HMGB1-TLR4在血清中水平与癫痫发病情况、药物治疗有效程度密切相关，可为癫痫发病机制研究提供新方向。
Objective To detect the high-mobility group box-1 （HMGB 1） and Toll-like receptor 4 （TLR4） levels in serum of epilepsy patients, and analyze their clinical significance. Methods Sixty-five epilepsy patients, admitted to our hospital from January 1,2011 to December 30, 2014, and 20 normal subjects as control group, were enrolled in our study; HMGB1 and TLR4 levels in serum of epilepsy patients were detected by double sandwich enzyme-linked immunosorbent assay. The relations of HMGB1 and TLR4 levels with efficiency degrees of drug therapy, mean time of epileptic seizure, seizure frequency, course of disease and epileptic seizure types were analyzed; the relation of HMGB 1 with TLR4 in serum was also analyzed. Results Levels of HMGB 1 and TLR4 in serum of epilepsy patients were significantly higher than those in normal controls （P〈0.05）. HMGB1 and TLR4 levels in serum of intractable epilepsy patients were statistically higher than those in drug-responsive epilepsy patients （/9〈0.05）. HMGB1 and TLR4 levels in patients whose epileptic seizures duration 〉5 rain were signficantly higher than those in patients whose epileptic seizures duration ≤ 5 min （P〈0.05）. HMGB1 and TLR4 levels in patients whose epileptic seizures frequency〉3/month were significantly higher than those of epileptic seizures frequency≤ 3/month （P〈0.05）. HMGB 1 and TLR4 levels in patients whose c ourse of seizure〉2 years were significantly higher than those in patients course of seizure ≤ 2 years （P〈0.05）. HMGB1 and TLR4 levels were not correlated with seizure types （P〉0.05）. Level of TLR4 in serum of epilepsy patients was positively correlated with HMGB1 level （r=0.926, P=-0.000）. Conelusion HMGB1 and TLR4 levels in epilepsy serum are closely correlated with epileptic seizures and response to antiepileptic drugs, and HMGB 1-TLR4 may become new target to study epilepsy.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2016年第9期936-940,共5页
Chinese Journal of Neuromedicine
基金
河南省重点科技攻关项目（092102310090）,郑州市科技领军人才项目（121PLJRC536）
