摘要
目的通过建立心肌梗死大鼠模型,进而且分子生物学手段检测心肌Egr-1和NF-κB的表达,以期为心梗医学鉴定提供更多的数据和更好的方法。方法 （1）通过手术建立心肌梗死大鼠模型;（2）应用半定量PCR和免疫组织化学法检测心肌组织内Egr-1的表达;（3）半定量PCR方法和weatern blotting检测p65表达。结果 （1）在术后1~7 d,有7只死于左心室破裂或急性心力衰竭。其梗死面积可达（44.2±1.8）%;（2）手术组的Egr-1相对表达量明显高于正常组和假手术组（P〈0.05）;（3）NF-κB P65在手术组中的表达量最高（P〈0.05）。结论在心肌梗死中有相同的刺激因表使得NF-κB和Erg-1被活化,从而使得其表达量上升为心肌梗死的诊断和预防提供条件。
Objective Objective to detecte the expression of Egr - 1 and NF - κB in myocardium ,the molecular biology methods were used .Methods （1 ）The myocardial infarction rat model was obtained by operation .（2 ） Egr - 1 expression level was detected by semi - quantity PCR and immunohistochemical .（3 ） NF - κB expression level was detected by semi - quantity PCR and western blotting .Result （1 ）Seven rats were died in left ventri-cle rupture or acute heart failure after operation from one day to seven days .（2 ）Compared with normal group and sham operation group ,the Egr - 1 expression level in operation group was higher （P 〈 0 .0 5 ） .（3 ） NF - κB P6 5 in operation group was highest （P 〈 0 .0 5 ） .Conclusion Egr - 1 and NF - κB were actived by same activator in myocardial infarction .
出处
《内蒙古医学杂志》
2016年第6期658-661,共4页
Inner Mongolia Medical Journal
