摘要
目的研究解酒中药复方"肝易复"(GYF)对小鼠急性酒精性肝损伤的保护作用并探讨其作用机制。方法预先灌胃给药21 d,末次给药后一次性灌胃体积分数50%的乙醇(12 m L·kg-1)造成急性酒精性肝损伤模型,8 h后取血、取肝。测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和超氧化物歧化酶(SOD)活性与丙二醛(MDA)水平;测定肝组织中SOD活性和MDA水平;HE染色观察小鼠肝脏组织病理学变化;RT-PCR技术检测肝脏中SOD、谷胱甘肽过氧化物酶(GPx)基因表达水平;氧化自由基吸收能力(Oxygen Radical Absorbance Capacity,ORAC)分析试验测定中药复方"肝易复"的抗氧化能力。结果 ORAC实验结果表明中药复方"肝易复"具有较好的抗氧化能力,并能降低血清中ALT和AST的水平(P<0.05,P<0.01),且能改善肝脏组织病理损伤,上调血清和肝组织中SOD的活性(P<0.05,P<0.01),降低血清和肝组织中MDA的水平(P<0.05,P<0.01),上调SOD和GPx的基因表达(P<0.05,P<0.01)。结论中药复方"肝易复"对急性酒精肝损伤小鼠具有一定的保护作用,其机制可能与增强肝脏抗氧化能力有关。
Objective To study the protective effects and mechanism of the Chinese herbal formula Ganyifu on theacute alcoholic liver injury in mice. Methods All mice except for the normal control group were treated with Ganyifitfor 21 days. After the last administration, 50 % ethanol at the concentration of 12m L·kg-1 was given to the medicatedmice while the normal control group received the same volume of distilled water. Eight hours later, blood and livertissue were sampled for the assay of serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST） andsuperoxide dismutase（SOD） activities as well as malondialdehyde（MDA） content. Meanwhile, hepatic SOD activitiesand MDA content were detected, and hepatic histopathologic changes were examined by HE staining. RT-PCR wasapplied to detect the mRNA expression of hepatic SOD and glutathione peroxidase（GSH-Px）. The antioxidant activity ofGanyifu was determined by oxygen radical absorbance capacity（ORAC） test in vitro. Results The results of ORAC testshowed that Ganyifu had certain antioxidant activity. Meanwhile, Ganyifu had an effect on decreasing serum ALT andAST levels （P 〈 0.05, P 〈 0.01） , relieving hepatic pathological injury, enhancing serum and hepatic SOD activity （P〈 0.05, P 〈 0.01） , decreasing serum and hepatic MDA content（P 〈 0.01 ）, and up-regulating gene expressions ofhepatic SOD and GPx as compared to the model group（P 〈 0.01 ）. Conclusion Ganyifu exerts certain protective effectson mice with acute alcoholic liver injury, and the mechanism may be related to the enhancement of antioxidant activity.
作者
毛中伏
谈硕彦
闫昌誉
黄晓雯
陈小梅
杨钦河
栗原博
何蓉蓉
李怡芳
MAO Zhongful TAN Shuoyan YAN Changyu HUANG Xiaowen CHEN Xiaomei YANG Qinhe KURIHARA Hiroshi HE Rongrong LI Yifang(Institute of Traditional Chinese Medicine and Natural Products, Pharmacy School, Jinan University, Guangzhou 510632 Guangdong, China The First Affiliated Hospital of Jinan University, Guangzhou 510632 Guangdong, China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2016年第5期660-665,共6页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广州市科技计划项目(2013J4501037)
关键词
中药复方
肝易复
急性酒精性肝损伤
抗氧化
Chinese herbal formula
Ganyifu
acute alcoholic liver injury
antioxidation
