摘要
为分析高山离子芥CbPLDβ基因的抗寒性价值,本实验构建了高山离子芥CbPLDβ的表达载体PBI121-CbPLDβ并转化烟草,经过筛选及PCR测序鉴定,获得转基因烟草植株.通过低温胁迫实验,分析了转基因烟草的抗寒性,结果表明:外源基因已经整合到烟草基因组中,在低温胁迫下转基因烟草植株的电解质渗透率普遍低于野生型烟草,而游离脯氨酸含量、可溶性糖和可溶性蛋白的含量均高于野生型烟草,说明CbPLDβ基因提高了烟草抵抗低温的能力.
In order to evaluate the function of CbPLDβ on cold tolerance of Chorispora bungeana, CbPLDβ was constructed into vector PBI121. The plant expression vectors PBI121-CbPLDβ were then transferred into tobacco using Agrobacterium-mediated transformation method. Transgenic tobacco lines were obtained after kanamycin screening and PCR detection. By low temperature experiments, the phys- iological and biochemical parameters were measured to study the cold resistance of CbPLDβ transgenic tobacco. The results showed that, the exogenous gene was integrated into the tobacco genome of trans- genic tobacco plants; the electrolyte leakage of transgenic tobacco is generally lower than the wild-type tobacco, and the free proline content, soluble sugar and soluble protein content were higher than the wild-type tobacco. These indicated that CbPLDβ improved the cold tolerance of tohacen.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第5期1113-1118,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(No.31160087
No.31360061)
