摘要
采用不同浓度的PEG6000(0%,5%,10%,15%,20%)模拟不同程度的干旱环境,通过测定第10 d,第15 d,第20 d栀子幼苗叶片内的抗氧化酶(SOD、POD、CAT)活性及可溶性蛋白含量等生理指标变化,了解栀子幼苗的耐干旱能力。结果表明:(1)相同处理时间内,POD、SOD活性和可溶性蛋白的含量随着PEG6000浓度的增加,呈现出先增后降的变化趋势;CAT活性在第10 d和15 d先增后降,第20 d CAT活性下降;PEG6000浓度为10%时,各指标数值最大。(2)相同胁迫强度,随着胁迫时间的增加,POD活性先增加后下降,在第15 d有最大酶活;SOD和可溶性蛋白的含量随胁迫时间的持续而下降;CAT活性在PEG6000浓度为5%和20%时下降,10%和15%时先升后降。(3)结论:较低浓度的PEG6000(≤10%)或较短的处理时间内(短于15 d)使栀子花幼苗生理活性增强;高浓度的PEG6000(≥15%)或较长的处理时间(长于15 d)使栀子幼苗生理活性下降。
Watering Gardenia seedlings with different concentrations（0,5%,10%,15%,and 20%） of PEG6000 which simulate different degrees of drought environment.On the 10 th,15th and 20 th day of watering some physiology indexes including of Antioxidant systems（POD,SOD,CAT） and content of the soluble protein were determined to know grought tolerance ability of Gardenia jasminoides Ellis.The resultes showed：（1） At the same time,following adding of PEG6000 concentration POD activity、SOD activity and concent of soluble protein increased firstly and then decreased;At the 10 th and the 15 th,CAT activity increased firstly and then decreased,but at the 20 th CAT activity decreased;when PEG6000 concentration is 10%,the index value were the most.（2）When PEG6000 concentration were the same,following extension of time POD activity increase firstly and then decrease,when POD activity was the most at the 15th;SOD activity and soluble protein concent decreased;CAT activity decreased when PEG6000 concentration were 5% and 20%,but CAT activity increased firstly and then decreased when PEG6000 concentration were 10% and 15%;（3）The conclusion：Treaed with lower concentration of PEG6000（≤10%） or less treating times（shorter than15 d）physiological vigor of Gardenia jasminoides Ellis. enhanced;Higher concentration of PEG6000（≥15%）or longer treating times（longer than 15d）physiological vigor of Gardenia jasminoides Ellis. reduced.
出处
《阜阳师范学院学报（自然科学版）》
2016年第3期41-45,共5页
Journal of Fuyang Normal University(Natural Science)
基金
阜阳师范学院自然科学研究重点项目(2015FSKJ03ZD)
省级平台委托专项课题(2014KSLZX01ZD)资助
关键词
栀子
干旱胁迫
生理特性
Gardenia jasminoides
drought stress
physiological characteristics
