摘要
为蚕蛹虫草的规模化生产提供技术支撑,以野生北冬虫夏草菌种为接种材料,以家养的活体桑蚕蛹为寄主,研究了蚕蛹虫草菌种制备、接种、发菌至出草的人工培养条件。结果表明：接种前削去蚕茧后放置2d,可有效去除病蛹及生命体征弱的蚕蛹,能在源头上控制污染;接种的蚕蛹虫草液体菌丝的活度越高,接种后的蚕蛹僵化越快;接种部位选择蚕蛹翼翅正后方与第3环节交叉点,易操作且蚕蛹体液和菌液不易溢出;菌丝完成营养生长后,给予300lx光照和10℃温差刺激（300lx光照22℃培养14h,黑暗13℃培养10h为周期）培养7~10d能有效促进蚕蛹虫草菌丝转色和子座的形成,在此条件下出草率达98.5%,虫草平均鲜重达1.25g。
The artificial rearing body of C. militaris were discussed conditions for strain preparation, by using living silkworm pupae inoculation, germination and fruiting as hosts and wild C. rnilitaris as an inoculation material to provide the technical support for large-scale production of C. militaris. Results： The broken silkworm cocoons for 2 d preparation before inoculation can efficiently eliminate silk pupae and poor life sign pupae and control contamination at source. The more the hyphal activity of inoculated C. militaris in the liquid medium is, the quicker the rigidity of inoculated silkworm pupae is. The optimum inoculation position is the intersection between the direct view behind wing and third link, which is easy operation and difficult slop over of silkworm cocoon body fluid and fungus fluid. The optimum culture conditions including 300 lx light intensity, 10℃ temperature difference （300 lx light intensity at 22℃ for 14 h and in dark at 13℃ for 10 h） and 7410 d culture can efficiently promote hyphal coloring and stroma formation of C. militaris after hyphae finish their vegetative growth. The germination rate and average fresh weight of C. militaris under the optimum culture conditions reaches 98.5 % and 1.25 g separately.
出处
《贵州农业科学》
CAS
2016年第7期65-68,共4页
Guizhou Agricultural Sciences
基金
四川省教育厅高校科研创新团队"川西珍稀植物保护与利用"(14TD0025)
内江师范学院2014年度大学生科研资助项目"蚕蛹虫草关键技术研究"(14NSD-38)
关键词
蚕蛹虫草
人工培养
出草率
鲜重
Cordyceps militaris
artificial rearing
germination rate
fresh weight
