摘要
建立了用凝胶渗透色谱-高效液相色谱测定茶叶中蒽醌的方法。以乙酸乙酯、乙酸乙酯-正己烷（1∶1,V/V）液液萃取,凝胶渗透色谱净化,收集时间为11~17 min,以甲醇＋0.5%H3PO4溶液（65＋35,V/V）为流动相,ZORBAX SB-C18分离,外标法定量。蒽醌质量浓度在10~200μg/L范围内线性关系良好,相关系数r为0.9993,检出限为6μg/kg,定量限为10μg/kg。茶叶样品在10,50,180μg/kg 3个加标浓度下,蒽醌的回收率为73.2%~97.5%,相对标准偏差为4.0~7.9%。
A method was developed for determination of anthraquinone in tea based on high performance liquid chromatography（HPLC） with gel permeation chromatography（GPC）. Sample was extracted with ethyl acetate,ethyl acetate- n-hexane（1： 1,V/V）,cleaned up with GPCwas and collected at 11 ~ 17 min. The analyte was then separated by HPLC ZORBAX SB-C18 column using methanol /0. 5% phosphoric acid（65： 35,V/V） as mobile phase,and quantified by the external standard method. The calibration curve was linear in the range of10 ~ 200 μg/L with correlation coefficient of 0. 9993. The limit of detection（LOD） was 6 μg/kg and the limit of quantitation（LOQ） was 10 μg/kg. Average recoveries of the method at three different spiked levels ranged from73. 2 % to 97. 5% with the relative standard deviation（RSD） from 4. 0% to 7. 9%.
出处
《分析试验室》
CAS
CSCD
北大核心
2016年第9期1083-1086,共4页
Chinese Journal of Analysis Laboratory
关键词
茶叶
蒽醌
凝胶渗透色谱
高效液相色谱仪
Tea
Anthraquinone
Gel permeation chromatography（GPC）
High performance liquid chromatography
