摘要
目的 探讨七氟烷对低温环境下失血性休克猪的心肌保护作用。方法 巴马小型猪32头,雌雄不限,体质量20～25 kg,按随机数字表法分为4组,每组8头。分别为假手术组（S组）、失血性休克组（HS组）、七氟烷预处理组（Pre/Sev组）和七氟烷后处理组（Post/Sev组）。监测并记录各组平均动脉压、心率和中心体温,各组分别于休克前及休克后30、60、90、120、180和240 min共7个时间点采集血液,采用ELISA法检测血清中白细胞介素-6（interleukin 6,IL-6）、肿瘤坏死因子-α（tumour necrosis factorα,TNF-α）和超氧化物歧化酶（superoxide dismutase,SOD）的含量。结果 与S组比较,HS组、Pre/Sev组和Post/Sev组血浆中TNF-α和IL-6含量在休克后60 min和90 min显著升高（P〈0.05）,其中TNF-α含量在休克120 min时达到高峰,休克180 min时显著低于120 min（P〈0.05）;血清中SOD含量显著降低（P〈0.05）。与HS组比较,Pre/Sev组和Post/Sev组血浆中TNF-α和IL-6含量显著降低（P〈0.05）;血清中SOD含量显著升高（P〈0.05）。Pre/Sev组和Post/Sev组各项比较差异无显著意义（P〉0.05）。结论 七氟烷对低温环境下失血性休克猪心肌具有保护作用,其主要机制与抑制炎症反应,增强机体抗氧化能力有关。
Objective To investigate the protective effect of sevoflurane on the myocardium of pig with hemorrhagic shock （HS）in environmental hypothermia. Methods Thirty-two Bama miniature pigs were randomly divided into four groups with eight animals for each group： sham group （group S）, hemorrhagic shock group （group HS ）, sevoflurane pre-conditioning group （group Pre/Sev ）and sevoflurane post- conditioning group（ group Post/Sev）. At the time points before HS （to ）, and 30,60,90,120,180 and 240 min after HS （ corresponding to t30 t60 t90 t120 t180 and t240 respectively ）, mean arterial pressure （ MAP）, heart rate （HR）and core temperature were monitored and recorded for every group, and at the same time, blood samples were collected, centrifuged and frozen until analysis for concentration of interleukin 6 （ IL-6 ）, tumour necrosis factor a （TNF-a） and superoxide dismutase （SOD） in serum. Results Compared with group S, the concentration of TNF-a and IL-6 in serum in group HS, Pre/Sev and Post/Sev was increased at 60 or 90 rain after HS（P 〈0. 05） and The TNF-α reached the maximum at 120 min after HS and decreased after HS 180 min（P 〈0. 05） while SOD in serum in group HS,Pre/Sev and Post/Sev decreased obviously（ P 〈 0. 05 ）. Compared with group HS ,TNF-α and IL-6 in serum of group Pre/Sev and Post/Sev was decreased （ P 〈 0.05 ） and SOD in serum of group Pre/Sev and Post/Sev was increased obviously （ P 〈 0.05 ）. There was no significant difference between group Pre/Sev and Post/Sev（ P 〉 0. 05 ）. Conclusions Sevoflurane has a protective effect on the myocardium of pig with hemorrhagic shock in environmental hypothermia, and the main mechanism may be related to attenuate inflammatory response and enhance the antioxidant capacity.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2016年第9期735-740,758,共7页
Journal of Shenyang Pharmaceutical University
基金
军队后勤科研重点资金资助项目(BWS12J008)
关键词
七氟烷
环境低温
失血性休克
心脏保护
白细胞介素-6
肿瘤坏死因子-α
氧化应激
小型猪
sevoflurane
environmental hypothermia
hemorrhagic shock
cardioprotective
interleukin 6
tumour necrosis factor or
oxidative stress
miniature pig
