摘要
目的用α_甲型溶血链球菌建立兔细菌性心内膜炎模型。方法自麻醉兔颈总动脉插一导管至左心室 ,24h后随机分为空白对照组和感染组。对照组耳缘静脉注射1ml生理盐水 ,感染组注射108CFU甲型溶血链球菌 ,7d后抽血进行血液培养 ,处死后观察主动脉瓣赘生物和左心室腔内壁性赘生物的情况。结果感染组的每个动物在主动脉瓣和左心室中底部均有大小不等的赘生物生长 ,血液中均有细菌。对照组则没有。结论用这种方法制作的兔实验性细菌性心内膜炎成功率高且简单易行。
Aim To establish the experimental rabbit model of endocarditis caused by α_hemolytic streptococcus.Methods A sterile polythene catherer was inserted through the right carotid artery across the aortic valve into the left ventricle.24h after the operation, the animals were radomly divided into two groups. Control group and infection group. In the infection group into each animal 108 CFU α_hemolytic streptococcus was injected through peripheral ear vein, while in the control group, I ml of normal saline was injected into each animal.7 d after injection, all animals were sacrified and the vegetations on aortic valve and endocardial wall of the left ventricle were removed, weighted, and the number of organisms in blood and vegetation was counted. Results Each infected animal had cardial valve vegetation and endocardial vegetation, there were organisms in blood and vegetation. Conclusion This method maked it easy to establish a rabbit endocarditis model.
基金
山东省自然科学基金青年基金资助课题
编号为Q94C0818
