摘要
研究北细辛种子生活力的快速测定方法,为北细辛种子利用及安全保存提供技术支撑。采用2,3,5-氯化三苯基四氮唑(TTC)染色法,设置不同的浸种时间、TTC浓度、染色温度、染色时间的正交试验,对北细辛种子生活力进行测定。影响北细辛种子生活力测定值大小的因素依次为TTC浓度>染色温度>染色时间>浸种时间;最佳条件:温水浸种6h、0.1%TTC溶液、30℃避光染色18h;最佳反应条件下测得种子生活力与发芽率实测值很相近。TTC法可作为北细辛种子生活力测定的标准方法,对北细辛栽培及种质资源保存具有重要意义。
The aim of the study is to study an appropriate method for testing seed viability of Asamm heterotropoides Fr. Schmidt vat. man＆ shuricum （Maxim.）Kitag. Using the 2,3,5- triphenyl tetrazolium chloride（TTC） method, the orthogonal test design set different soaking time, different TTC concentration, different dyeing temperature, and different dyeing time were detected to research seed viability measurement conditions. Tne optimum conditions for testing seed viability were soaking seeds for 6h and then staining in 0.1% TTC solution at 30℃ for 18h. Under these conditions seed viability is significantly related to gen＇nination. Research the optimum conditions for testing seed viability, It has great significance in cultivation and gertnplasm conservation. TIC method could be used as the standard method for testing seed viability.
出处
《特产研究》
2016年第3期31-35,共5页
Special Wild Economic Animal and Plant Research
基金
国家科技重大专项(2009ZX09308,2012ZX09304006)
国家中医药管理局国家公共卫生专项
吉林省质量技术监督局项目(10043)
关键词
北细辛
2
3
5-氯化三苯基四氮唑法
种子生活力
Asarum heterotropoides F. Schmidt var. mandshur/cum （Maxim.） Kitag.
TIC Method
seed viability
