摘要
目的:建立同时测定3种近缘植物仙鹤草、委陵菜和翻白草中芦丁、金丝桃苷、木犀草苷、槲皮素、芹菜素、山柰酚6种黄酮类化合物含量的UHPLC法,并比较3种药用植物鲜品和干品中6种黄酮类化合物的含量。方法:样品用70%乙醇超声提取0.5 h,采用Acquity HSS T3柱(100 mm×3.0 mm,1.8μm),以0.3%冰乙酸溶液(A)-乙腈(B)为流动相,梯度洗脱,流速为0.4 m L/min,检测波长为360 nm,柱温为35℃。结果:3种植物干品中,芦丁和金丝桃苷的含量普遍较高,山柰酚含量极低;委陵菜中芹菜素含量达到0.028 mg/g,翻白草和仙鹤草中未检测出芹菜素;仙鹤草中木犀草苷含量较高,而翻白草和委陵菜中木犀草苷含量较低。委陵菜和翻白草的鲜品中6种黄酮总含量高于干品,而仙鹤草反之。结论:3种近缘植物中委陵菜和翻白草的鲜品与干品黄酮含量变化趋势相同,从化学分类角度证实两者更为近缘。该方法简单、快速且高效,应用该方法分析3种近缘植物中黄酮类成分的含量差异,为临床合理用药提供了科学依据。
Objective： To establish an UHPLC method for simultaneous determination of six flavonoid components of three closelyrelated plants Agrimonia pilosa,Potentilla chinensis and Potentilla discolor including rutin,hyperoside,cynaroside,quercetin,apigenin and kaempferol. Meanwhile three fresh and dry plants were evaluated to compare the contents of six flavonoid components. Methods： The samples were pretreated with ultrasonic extraction with 70% ethanol for 0. 5 h. The analysis was performed on an Acquity HSS T3（ 100 mm × 3. 0 mm,1. 8 μm） column with the mobile phase consisting of acetonitrile and 0. 3% glacial acetic acid aqueous at a flow rate of0. 4 m L / min. The detection wavelength was 360 nm,and the column temperature was 35 ℃. Results： The contents of rutin and hyperoside were high generally,but the content of kaempferol was extremely low in three closely-related plants. The content of apigenin was0. 028 mg / g in Potentilla chinensis,but not detected in Agrimonia pilosa and Potentilla discolor. The content of cymaroside in Agrimonia pilosa was significantly higher than that in Potentilla chinensis and Potentilla discolor. The fresh plants of Potentilla chinensis and Potentilla discolor contained more flavonoids than oven drying plants. Conclusion： The similar trend of content change from fresh to dry plant has showed a chemotaxonomic relationship of Potentilla chinensis and Potentilla discolor. The established determination method is simple,rapid and efficient,and is applicable for analysis of the contents of flavonoids in three closely-related plants,which provides the scientific basis for rationalization of using these drugs in clinic.
出处
《中药材》
CAS
CSCD
北大核心
2016年第5期991-995,共5页
Journal of Chinese Medicinal Materials
基金
天津市应用基础与前沿技术研究计划(重点项目)(14JCZDJC37000)
关键词
仙鹤草
委陵菜
翻白草
黄酮
含量测定
UHPLC
Agrimonia pilosa Ledeb
Potentilla chinensis Ser
Potentilla discolor Bge
Flavonoid
Contents determination
UHPLC
