摘要
目的:研究接种AM真菌后滇重楼根际土壤微生物和酶活性的变化规律,为人工栽培滇重楼提供技术基础。方法:通过室温盆栽接种试验与室内分析相结合的方法,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对滇重楼根际土壤微生物和土壤酶活性的影响。结果:滇重楼与AM真菌之间存在一定的相互选择性。AM真菌诱导处理后,不同AM真菌对滇重楼根际土壤微生物的数量、微生物生物量碳和土壤酶活性的影响有所差异,AM真菌的施用降低了滇重楼根际土壤中可培养真菌的数量,而对可培养细菌、放线菌数量呈现出增加的趋势,并提高了根际土壤微生物多样性指数、微生物生物量碳含量以及蛋白酶、脲酶、磷酸酶、过氧化氢酶和蔗糖酶活性。结论:滇重楼人工栽培中施用AM真菌能促使滇重楼根际微生物区系从低肥力的"真菌型"向高肥力的"细菌型"转化的趋势,提高根际土壤微生物多样性和酶活性,有助于维持滇重楼根际微生态系统的稳定性与和谐性,证明了田间人工栽培滇重楼施加AM菌剂的有效性及可能性。
Objective： To investigate the rhizosphere soil microorganism and enzyme activity after Paris polyphylla var. yunnanensis inoculated Arbuscular Mycorrhizal（ AM） fungi,which provide the technological condition for artificial cultivation of Paris polyphylla var. yunnanensis. Methods： By the method of combining the greenhouse pot inoculation trials with laboratory analysis,The effects of inoculation with 28 AM fungi on rhizosphere soil microorganism and soil enzyme activities of Paris polyphylla var. yunnanensis under sterilized soil conditions were observed. Results： There were certain mutualistic selection between Paris polyphylla var. yunnanensis and AM fungi. After induction treatment with AM fungi,different AM fungi had various effects on the quantity,microbial biomass carbon and soil enzyme activities of rhizosphere soil microorganism of Paris polyphylla var. yunnanensis. With application of AM fungi,the amount of culturable fungi from rhizosphere soil of Paris polyphylla var. yunnanensis were decreased,while the culturable bacteria and actinomycetes showed a rising trend,and increased the diversity index,the content of biomass carbon and protease,urease,phosphatase,catalase and invertase activities of rhizosphere soil microorganism. Conclusion： Applying AM fungi when artificial cultivate Paris polyphylla var. yunnanensis,by which can impel rhizosphere microorganism of Paris polyphylla var. yunnanensis to transform from low-fertile＂fungus types＂to high-fertile＂bacterial forms＂,and raise fungi diversity and enzymatic activity of rhizosphere soil microorganism,it is helpful to maintain the stability and harmoniousness of microecosystem on rhizosphere of Paris polyphylla var. yunnanensis,which certify the efficiency and the possibility of spreading AM inoculum during artificial cultivation in the field.
出处
《中药材》
CAS
CSCD
北大核心
2016年第5期948-955,共8页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81260622
41301248)
云南省应用基础研究计划(2011FB081)
云南省教育厅科学研究基金(2012Z119)
关键词
AM真菌
滇重楼
土壤微生物
土壤酶活性
微生物量碳
AM fungi
Paris polyphylla Smith var
yunnanensis（Franch
） Hand
-Mazz
Soil microorganism
Soil enzyme activity
Microbial biomass carbon
