摘要
目的通过比较膜突蛋白和上皮细胞钙黏蛋白（E-cadherin）在非特殊型乳腺浸润性癌（BIC-NST）、乳腺导管原位癌（BDCIS）及癌旁正常组织中的表达，探讨膜突蛋白和E-cadherin与乳腺癌临床病理参数的相关性。方法收集惠州市中心人民医院2008年1月至2010年12月乳腺癌病例标本，采用组织芯片免疫组织化学SP法，检测104例BIC-NST、84例BDCIS及53例癌旁组织中膜突蛋白和E-cadherin的表达；采用Western blot检测16例新鲜BIC-NST组织的膜突蛋白表达。结果膜突蛋白在BIC-NST及BDCIS组织中的表达明显高于癌旁组织（P〈0.01），而E-cadherin在BIC-NST及BDCIS组织中的表达明显低于癌旁组织（P〈0.01）；膜突蛋白在BIC-NSTⅢ级分化组的表达率明显高于Ⅰ级分化组（P〈0.05），而且组织分级越高，膜突蛋白表达越高，呈明显的正相关（P〈0.05），但E-cadherin在BIC-NST Ⅲ级分化组的表达率明显低于Ⅰ级分化组（P〈0.05），而且组织分级越高，E-cadherin表达越低，呈明显的负相关（P〈0.05）。在BDCIS组织中膜突蛋白及E-cadherin表达与分级不相关。膜突蛋白表达在患者临床分期Ⅲ＋Ⅳ期中的表达明显高于Ⅰ＋Ⅱ期的表达（P〈0.01），与淋巴结转移相关（P〈0.01），但与年龄、肿物大小及脉管浸润不相关；E-cadherin表达在患者临床分期Ⅲ＋Ⅳ期中的表达明显低于Ⅰ＋Ⅱ期的表达（P〈0.01），与淋巴结转移及脉管浸润相关（P〈0.01），但与年龄、肿物大小不相关；膜突蛋白及E-cadherin在BIC-NST（P＝0.021）及BDCIS（P＝0.032）中的表达呈负相关。结论膜突蛋白信号的高表达及E-cadherin信号的低表达与乳腺癌的发生发展密切相关，可为乳腺癌的基因治疗提供新的靶点。
Objective To investigate the correlation of moesin and E-cadherin with biological behavior of breast cancer and its mechanism by comparing expression of moesin and E-cadherin in breast invasive carcinoma of no specific type （BIC-NST） , breast duetal carcinoma in situ （ BDCIS ） and normal breast tissues adjacent to carcinoma. Methods Breast cancer cases of the Huizhou Municipal Center People Hospital were collected between Jan 2008 and Dec 2010, expression of moesin and E-cadherin in 104 cases of BIC-NST,84 cases of BDCIS and 53 cases of normal breast tissues adjacent to carcinoma were detected by tissue-microarray and SP immunohistochemical staining. Western blot was used to detect moesin expression of 16 BIC-NST fresh tissues. Results Expression rate of moesin in BIC-NST and BDCIS were significantly higher than normal tissues （ P 〈 0.01 ）, but the expression rate of E-eadherin in BIC-NST and BDCIS were significantly lower than those of normal tissues（ P 〈 0.01 ）. Expression rate of moesin in BIC-NST grade Ⅲ group was significantly higher than that of the grade I group. There was a significantly positive correlation between histological grade and moesin expression （P 〈 0. 05 ）. However, E-eadherin expression rate in BIC- NST grade Ⅲ group was significantly lower than that in grade I group , and there was a significantly negative correlation between histological grade and E-eadherin expression （P 〈 0. 05 ）. Moreover, no significant correlation was observed between moesin and E-cadherin expression in BDCIS tissues. Expression of moesin in clinical stage Ⅱ＋ Ⅲ BIC-NST was significantly higher than that in stage I （ P 〈 0.01 ） . Expression of moesin was significantly associated with lymph node metastasis （P 〈0. 01 ）. But no significant correlation was observed between moesin expression and age, tumor size and vascular invasion . However, expression of E-cadherin in clinical stage Ⅱ ＋ Ⅲ BIC-NST was significantly lower than that in stage Ⅰ （P 〈0. 01 ）. Expression of E-cadherin was significantly associated with lymph node metastasis and vascular invasion （P 〈 0. 01 ）. But no significant correlation was observed between E-cadherin expression, age and tumor size. There was a negative correlation between expression of moesin and E-cadherin in BIC-NST（P = 0. 021 ）and BDCIS（P = 0. 032）. Conclusion Higher moesin and lower E-cadherin signal transduction is closely related to the recurrence and development of breast carcinoma, therefore moesin and E-cadherin might provide new targets for gene therapy in breast carcinoma.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2016年第8期550-555,共6页
Chinese Journal of Pathology
基金
惠州市科技计划项目（惠市科字[2013]2013Y032）
