摘要
目的:探讨温肾醒脑方对VD模型大鼠学习记忆功能、神经干细胞增殖及b FGF基因表达的影响,阐明温肾醒脑方益智醒脑开窍的机理。方法:采用"劳倦过度、房室不节"法、高脂饮食法以及反复性脑缺血再灌注结合降压法复制肾(阳)虚痰瘀型VD模型,将动物随机分为正常组、假手术组、模型组、温肾醒脑方组(中药组),于造模后1 d、30 d处死动物,评价动物的认知功能,免疫组织化学法观察nestin阳性细胞,ELISA和RT-PCR检测b FGF表达水平。结果:造模后动物逃避潜伏期明显延长,中药治疗组与模型组相比较,逃避潜伏期时间明显缩短差异具有统计学意义(P<0.05);中药组与假手术组相比,差异无显著性(P>0.05)。正常大鼠海马和皮层有少量nestin阳性细胞,造模后假手术组、模型组和中药组大鼠nestin阳性细胞数稍有增加,差异无统计学意义(P>0.05);30天后模型组、假手术组与正常组nestin阳性细胞数基本相近,差异无统计学意义(P>0.05),中药治疗组在海马与皮层均有显著增加,与正常组、模型组相比差异具有统计学意义(P<0.05)。正常组脑组织有低水平b FGF蛋白和基因表达,造模后模型组、假手术组与中药治疗组表达增强,中药治疗组、模型组与正常组相比差异有统计学意义(P<0.05),但两组间差异无统计学意义(P>0.05);中药治疗组b FGF水平维持在比较高的水平,而模型组基本恢复到正常组水平,中药治疗组与模型组、正常组相比差异均有统计学意义(P<0.05)。结论:温肾醒脑方能促进认知功能的恢复,增强VD大鼠神经干细胞增殖和b FGF表达,是其益智醒脑开窍的可能机理。
Objective： To explore the effect of Wenshen Xingnao Decoction on cognitive function and neural stem cells and b FGF in VD rats and make the mechanism clear. Methods： The over- fatigue and sexual method,high fat diet and repeated cerebral ischemia reperfusion with step- down method were used to duplicate the kidney yang deficiency with phlegm and blood stasis type of vascular dementia model. The animals were randomly divided into normal group,sham- operated group,model group,the Chinese medicine group. And then on 1 d and 30 d,animals were killed to evaluate the cognitive function and nestin- positive cells were observed by immunohistochemical method and b FGF assayed by ELISA and RT- PCR. Results： After modeling,animal escape latency was significantly longer and there was significant difference between the model group and Wenshen Xingnao Decoction group at 30 d（ P 〈 0. 05）. There was no significant differences between same- operated group with Wenshen Xingnao Decoction group（ P 〉 0. 05）. There was a certain amount expression of nestin in normal brain tissue. The nestin- positive cells were increased in model group and the Chinese medicine group. There was significant difference between the model group and Wenshen Xingnao Decoction group at 30 d（ P 〈 0. 05）. There was no significant differences between same- operated group and Wenshen Xingnao Decoction group（ P 〉 0. 05）. In the normal and sham- operated groups,rats brains have a low level of b FGF which were increased in model group and the Chinese medicine group on 30 d. There was significant difference between the model group and Wenshen Xingnao Decoction group on 30 d（ P 〈 0. 05）. There was no significant differences between same- operated group and Wenshen Xingnao Decoction group（ P 〉 0. 05）. Conclusion： Wenshen Xingnao Decoction can promote the recovery of cognitive function,enhance VD rats neural stem cell proliferation and the expression of b FGF,which may be its mechanism of benefiting the intelligence and opening the orifices.
出处
《辽宁中医杂志》
CAS
北大核心
2016年第8期1741-1744,I0001,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(U1504826)
河南省重点科技攻关项目(152102310224)
关键词
温肾醒脑方
血管性痴呆
认知功能
神经干细胞
BFGF
Wenshen Xingnao Decoction
vascular dementia
cognitive function
neural stem cells
bFGF
