摘要
南方夏季的高温胁迫,使芍药茎叶因热害提早枯萎,并导致地下块根更新发育不良,是"芍药南移"所面临的主要困难。为探讨南方夏季高温胁迫下芍药的生理生化响应,比较耐热性评价方法,以浙江本土药用植物杭白芍和9个菏泽的观赏品种为材料,对夏季高温下的芍药茎叶进行热害指数观测,测定叶片的相对电导率、相对含水量、叶绿素含量、可溶性糖含量、丙二醛含量、可溶性蛋白含量、抗坏血酸过氧化物酶活性和超氧化物歧化酶活性;并利用隶属函数法、隶属函数法结合主成分分析2种方法对10个品种的耐热性展开评价。结果表明,杭白芍、杨妃出浴和春晓具备较好的耐热性,适合江南地区选用;隶属函数法是芍药耐热评价的简便、直观、有效的评价方法。本研究结果为"芍药南移"及抗性育种研究提供了重要参考。
In summer time, the high temperature in south region of the Yangzi River （eg. Zhejiang Province） is the most severe stress for landscape use of P. lactiflora, which usually causes serious heat injury and results in the degradation of tuberous root year by year. To elucidate the physiological and biochemical responses, compare different heat resistance evaluation methods of P. lactiflora under high temperature stress in the south of China, we investigated the heat injury index （HII） for stem and leaf of one medicinal cultivar named Hangbaishao planted in Zhejiang Province, and nine ornamental cultivars introduced from Heze City of Shandong Province in this research. The physiological and biochemical levels of relative electrical conductivity （REC） , relative water content （RWC） , chlorophyll content （CC） , soluble sugar content （ SSC ）, methane dicarboxylic aldehyde content （ MDA）, soluble protein content （ SPC ）, ascorbate peroxidase activity （APX） and superoxide dismutase activity （SOD） were measured. Based on these data of eight indices, we adopted the subordination function method （also named membership function method, SF method for short in this article） , and the combination of subordination function method and principal component analysis （ SF ＋ PCA method for short in this article）, to evaluate the heat tolerance of ten different cultivars and comparison of this two assessment methods was involved as well. The results indicated that Hangbaishao, Yangfei Chuyu and Chunxiao had the strongest heat resistance, both of which were suitable for landscape using in south region of the Yangzi River. The SF method was confirmed to be a simple and efficient method to evaluate the heat resistance of herbaceous peony. The results provide an important reference for the study of P. lactiflora southward and resistance breeding.
出处
《核农学报》
CAS
CSCD
北大核心
2016年第9期1848-1856,共9页
Journal of Nuclear Agricultural Sciences
基金
浙江省花卉新品种选育重大科技专项重点项目(2012C12909-16)
关键词
芍药
高温胁迫
生理生化响应
耐热评价
隶属函数法
主成分分析
Paeonia lactiflora Pall. , high temperature stress, physiological and biochemical responses, heat resistance evaluation, subordination function method, principal component analysis
