摘要
为了制备高吸附性和特异性的倍硫磷分子印迹聚合物,采用表面印迹技术,以硅胶为载体,倍硫磷为模板分子,合成的N,O-双异丁烯酰丝氨醇为功能单体,制备倍硫磷分子印迹聚合物;并通过傅里叶红外光谱、场发射扫描电镜和吸附试验对其性能进行研究。结果表明,在硅胶载体表面成功地接枝了倍硫磷印迹聚合物,印迹聚合物存在2类不同的结合位点,平衡离解常数(Kd)和最大表观结合量(Qmax)分别为K_(d1)=0.282μg·mL^(-1)、Q_(max1)=1.071μg·mg^(-1)、K_(d2)=0.669μg·m L^(-1)、Q_(max2)=0.483μg·mg^(-1),对倍硫磷的吸附在60~80 min内达到平衡。该印迹聚合物对倍硫磷具有选择性识别性能,可用于固相萃取介质,这为食品中倍硫磷的残留检测奠定了研究基础。
In order to prepare high adsorptive and specific fenthion molecularly imprinted polymers, a series of molecularly imprinted polymers were synthesized on the surface of modified silica, using fenthion as template molecule, synthesized N,O-bismethacryloyl serinol as functional monomer. The FT-MIPs was characterized by Fourier transform infrared spectroscopy, scanning electron microscopic and adsorption experiments. The results showed that a layer of FT- MIPs was successfully grafted on the surface of silica gel carrier. There were two different kinds of binding sites in FT- MIPs, the equilibrium dissociation constant （ Kd） and apparent maximum binding capacity （Qmax） of high affinity sites and low affinity sites were Kd1=0.282μg·mL^-1、Qmax1=1.071μg·mg^-1、Kd2=0.669μg·m L^-1、Qmax2=0.483μg·mg^-1 , respectively. The FT-MIPs reached the adsorption equilibrium for FT within 60 - 80 min. The FT-MIPs had selective recognition property for FT and was expected to be used as solid phase extraction medium, which would provide research basis for detecting fenthion residue in food.
出处
《核农学报》
CAS
CSCD
北大核心
2016年第9期1744-1753,共10页
Journal of Nuclear Agricultural Sciences
基金
河南省教育厅科学技术研究重点项目(14B550015)
关键词
倍硫磷
N
O-双异丁烯酰丝氨醇
分子印迹聚合物
表面印迹技术
正交试验
fenthion, N, O-bismethacryloyl serinol, molecularly imprinted polymers, surface molecular imprinting technology, orthogonal experiment
