摘要
为调查达州、广安、攀枝花、巴中、资阳等四川省主要山羊产业区内的山羊皮下脓肿病的流行病学和致病病原,并对临床分离菌进行药物敏感性评价,以指导合理使用抗生素。在厌氧和常规培养条件下从临床病例中分离细菌,结合细菌的菌落形态和细菌生化特性分析,通过16Sr RNA基因序列分析进行细菌鉴定;测定分离菌的半数致死量（LD50）以判定其毒力强弱;使用CLSI推荐的K-B法进行药敏试验。结果表明,该病以1周岁左右的母山羊在春秋季发病为主,发病率为8.36%-11.27%,平均发病率为9.44%;分离的主要致病菌是伪结核棒状杆菌和金黄色葡萄球菌;其中伪结核棒状杆菌xw2株的LD50为107.5CFU/m L,金黄色葡萄球菌xj5株的LD50为109.7CFU/m L;药敏试验发现这两种分离菌的耐药现象比较普遍,对青霉素和庆大霉素的耐药现象比较严重,但20株伪结核棒状杆菌菌株对环丙沙星、利福平、头孢噻污的敏感率均≥85.00%,14株金黄色葡萄球菌菌株对氨苄西林、左氧氟沙星、利福平的敏感率均≥85.71%。
The study aims to investigate the epidemiology and pathogens of the subcutaneous abscess cases distributed in the main goat industry of Dazhou,Guang＇an,Panzhihua,Bazhong and Ziyang districts in Sichuanprovince,and to estimate drug susceptibility of the clinical isolates of pathogens so as to guide the reasonable use of antibiotics. The bacteria were isolated from the clinical cases in the conditions of anaerobic and conventional culture,and were identified based on its colony morphology, biochemical properties and 16S rRNA gene sequence analysis. The virulence of the isolates was deter- mined by means of median lethal dose（LD50）,and drug susceptibility testing was performed by K-B method recommended by CLSI. The results showed that the disease mainly spread in the herds of one year-old goats in the Spring and Autumn season,and the incidence rate of the disease was 8.36%--ll.27%,and the average incidence rate was 9.44%.The bacteria isolates ranked the top 2 species of pathogens were Corynebacterium pseudotuberculosis and Staphylococcus aureus,and among these isolates,LDb0 of xw2 of C. pseudotuberculosis was 10^7.5 CFU/mL,and the LD50 of xj5 of S. aureus was 10^9.7 CFU/mL. It was common that C. pseudotuberculosis and S. aureus were resistant to penicillin and gentamicin by drug suscepti- bility testing. Among 20 isolates of pseudotuberculosis,the drug susceptibility rates of C. pseudotuberculosis to ciprofloxacin,rifampicin and cefotaxime were more than 85.00%;among 14 isolates of S. aureus,the drug susceptibility rates of S. aureus to ampicillin,levofloxacin and rifampicin were more than 85.71-.The study analyzed the main pathogens from goats with subcutaneous abscess in Sichuan province and provides a reference for the prevention and control of the diseases.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2016年第8期991-996,共6页
Chinese Veterinary Science
基金
四川农业大学创新性实验计划项目(1510626057)
关键词
山羊皮下脓肿
流行病学调查
病原分离
16Sr
RNA
半数致死量
药敏试验
goat subcutaneous abscess
epidemiological investigation
pathogen isolation
16SrRNA
median lethal dose
drug susceptibility testing
