摘要
目的:通过响应面法优化复合酶提取半夏多糖的工艺,并评价其抗氧化活性。方法:以半夏多糖得率为响应值,以液料比、酶解温度、酶解时间、复合酶(木瓜蛋白酶、纤维素酶、果胶酶的质量比为2∶2∶1)添加量为试验因素,采用响应面法建立数学模型,优化提取条件;体外抗氧化活性考察半夏多糖对DPPH和O_2^-·自由基的清除能力。结果:通过二次回归模型响应面分析,酶解温度、时间、复合酶添加量、液料比4因素对半夏多糖得率的影响依次减弱;酶解温度与时间优化为54℃和57分钟,复合酶添加量为7.2 mg/mL,液料比例为27∶1 mL/g,在此最佳工艺条件下半夏多糖得率为27.98%,模型方程理论预测值为28.32%,两者相对误差小于5%。半夏多糖具有较强的抗氧化活性,对DPPH和O_2^-·自由基的半数抑制浓度分别为0.987 mg/m L、1.309mg/m L,但与维生素C比较,抗氧化活性较弱。结论:采用响应面法优化得到了半夏多糖的最佳复合酶提取条件,且半夏多糖有一定的体外抗氧化作用。
Objective： To optimize complex enzymatic extraction technology of polysaccharide from BanXia （Pinellia temata） by response surface method, and evaluate its antioxidant activity. Methods： Polysaccharide yield ofBanXia was taken as the response, liquid to material ratio, enzymatic hydrolysis temperature, enzymatic hydrolysis time and complex enzymes （mass ratios of papain, cellulose and pectase were 2：2：1） as experimental factors, mathematical model was established by adopting response surface method to optimize extraction conditions; antioxidant activity in vitro was used to investigate the clearance ability ofpolysaccharide extracted from BanXia to DPPH and O_2^-·radical. Results： By analyzing with two regression model response surface, the influences of enzymatic hydrolysis temperature, enzymatic hydrolysis time, additive amount of complex enzymes and liquid to material ratio became attenuated gradually; enzymatic hydrolysis temperature and enzymatic hydrolysis time were optimized as 54℃ and 57 minutes, additive amount of complex enzymes were 7.2 mg/mL, liquid to material ratio was 27：1 mL/g, under the best extraction technology, polysaccharide yield of BanXia was 27.98%, model equation theoretical prediction value was 28.32%, and relative error between both was less than 5%. Polysaccharide from BanXia presented stronger antioxidant activity, half maximal inhibitory concentrations of DPPH and O2-radical were 0.987 mg/mL and 1.309 mg/mL, but its antioxidant activity was weaker compared with vitamin C. Conclusion： The best complex enzymatic extraction technology of polysaccharide from BanXia is obtained by adopting response surface method, and the polysaccharide from BanXia shows antioxidant activity in vitro to a certain degree.
出处
《西部中医药》
2016年第7期37-41,共5页
Western Journal of Traditional Chinese Medicine
关键词
半夏
多糖
复合酶
响应面
抗氧化
BanXia
polysaccharide
complex enzyme
response surface
antioxidation
