摘要
目的研究创伤后应激障碍(PTSD)大鼠血浆、海马CA_1区和齿状回神经元内脑源性生长因子(BDNF)的变化。方法用单一连续刺激(SPS)方法刺激大鼠产生PTSD模型,另进行强迫游泳(FS)刺激作为对照,ELISA检测不同时间(正常、刺激后2 h、12 h、1 d、7 d以及7 d后再次给予强迫游泳后2 h)大鼠血浆BDNF;取SPS后2 h、7 d、SPS+再次游泳后2 h和FS+再次游泳后2 h鼠脑组织,正常脑组织为对照,免疫组织化学技术观察大鼠海马神经元内BDNF的表达,以及采用荧光实时定量PCR法检测大鼠海马神经元内的BDNF-mRNA相对表达。结果大鼠经SPS刺激后2 h时血浆BDNF明显高于正常,7 d时与正常大鼠无明显差异,SPS+再游泳-2 h时明显高于各时间段及FS+再游泳后2 h;大鼠海马CA1区、齿状回内BDNF的表达以及海马内BDNF-mRNA相对表达也出现相似的改变。结论 PTSD大鼠血浆中BDNF浓度变化与海马内BDNF表达相关,BDNF的改变影响PTSD大鼠对创伤刺激的恐惧记忆形成、巩固和再摄取。
Objective To study the changes of brain-derived growth factor （ BDNF） in plasmahippocampal CA2and dentate gyrus of PTSD-rats. Methods Used the single prolonged stress （SPS） method to stimulate the rat in order toset up PTSD model of rat, and the normal rats were stimulated by forced swimming （ FS） as control group. Detected theplasma BDNF of rats at different time points （normal,2h、 12 h、1 d、7 d after stimulating, and 2 h after re-forced swimming7 days later） by ELISA; In addition, the brain of rats were got in 2 h、7 d after SPS,2 h after SPS ＋ re-forced swimming,2 hafter FS ＋ re-forced swimming and the rat brain of control group. The expression of BDNF in hippocampal neurons in ratswas observed with immunohistochemical technique; Real time fluorescence quantitative PCR （RT-PCR） was used to detectthe relative expression of BDNF-mRNA in hippocampus of rats. Results Rats’ plasma BDNF in SPS-2 h was significantlyhigher than normal rats. It was back in SPS-7 d,and it had no obvious difference with the normal rats. The concentration ofBDNF in SPS ＋ re-forced swimming-2 h was significantly higher than others experimental periods and FS ＋ re-forced swimming-2 h. The expression of BDNF in rats5 hippocampal CA2 ,dentate gyms and the relative expression of BDNF-mRNA inthe hippocampus also appeared the similar change like it in plasma. Conclusion The changes of BDNF concentration inPTSD-rats5 plasma are associated with the expression of BDNF in the hippocampus. The changes of BDNF result the rats toproduce fear memory after they have been suffered a traumatic stimuli,and the memory can be consolidated and re-extractedeasily.
出处
《中风与神经疾病杂志》
CAS
北大核心
2016年第8期715-718,共4页
Journal of Apoplexy and Nervous Diseases
基金
辽宁省科技厅科学技术计划(No.2011225020)
关键词
创伤后应激障碍
单一连续刺激
海马
脑源性生长因子
Post-traumatic stress disorder
Single prolonged stress
Hippocampus
Brain-derived growth factor
