摘要
目的:观察5-羟色胺2B受体(5-HTR2B)、E-钙粘素(E-cad)、α-平滑肌蛋白(α-SMA)在博莱霉素(BLM)致大鼠肺纤维化中的表达变化。方法:健康雄性SD大鼠45只,随机分为3组(n=15):对照组、BLM组、泼尼松+BLM组。各组分别于第7、14和28天各处死动物5只,取肺组织,行HE、Masson染色,观察大鼠肺组织炎症和纤维化的程度;免疫组化法及逆转录-聚合酶链反应(RT-PCR)法检测肺组织中5-HTR2B、上皮细胞标记物E-cad和间质细胞标记物(α-SMA)mRNA及蛋白的表达水平。结果:BLM组肺组织病理切片HE和Masson染色按时间顺序呈现由肺泡炎症至纤维化的动态改变。免疫组化及RT-PCR结果显示肺纤维化大鼠肺组织中5-HTR2B、α-SMA的蛋白及mRNA表达均增加(P<0.05),28 d时最高;E-cad蛋白及mRNA表达均减弱(P<0.05),28 d时最低;泼尼松+BLM组,相应时间点5-HTR2B、α-SMA蛋白及mRNA表达有所下降(P<0.05),E-cad蛋白及mRNA表达不同程度增强(P<0.05)。结论:5-HTR2B可促进大鼠肺纤维化发生,机制可能与减弱E-cad,促进α-SMA表达,诱导上皮细胞间质转化(EMT)有关。
Objective： To observe the expression of 5-Hydroxytryptamine 2B receptor （5-HTR2B）, E-cadherin （E-cad）, alpha-smooth muscle actin（a-SMA） in the bleomycin -induced pulmonary fibrosis tissue of rats. Methods： Forty-five healthy male SD rats were randomly divided into control group, bleomycin group and bleomycin ＋ prednisone group（ n = 15 ）. Five rats in each group randomly sacrificed at the 7^th, the 14^th and the 28^th day after eastablishing models. The lung tissue was observed by microscope in HE and Masson staining. Lung hydroxyproline （HYP） content was evaluated. The expression of protein and mRNA of 5-H3R2B, E-cad and α-SMA were analyzed by immunohistocbemistry and/or RT-PCR. Results： A dynamic changes from alveolitis to pulmonary fibrosis could be observed in the slices by HE and Masson staining. The experimental results by immunohistochemistry and RT-PCR showed that the protein and mRNA expression of 5-HTR2B and a- SMA enhanced rat pulmonary fibrosis （P 〈 0.05）and reached the highest at the 28th day; the corresponding protein and mRNA expression of E-cad reduced （ P 〈 0.05）, and reduced to the lowest value at the 28^th day. Compared with bleomycin group, the corresponding mRNA and protein expression of 5-HTR2B and α-SMA in bleomycin ＋ prednisone group had decreased （P 〈 0.05）; however, the corresponding mRNA and protein expression of E-cad had increased（ P 〈 0.05）. Conclusion： 5-HTR2B is involved in the pathogenesis of pulmonary fibrosis throught epithelial- mesenchymal transition （EMT）.
出处
《中国应用生理学杂志》
CAS
CSCD
2016年第4期365-369,I0009,I0010,共7页
Chinese Journal of Applied Physiology
