摘要
体外诱导方法制备含有抗菌活性物质的柞蚕蛹粉,并将其以不同比例(0%、1.0%、2.0%)添加到刺参饲料中,并对照商品抗菌肽产品(0.5%添加),制成C、M1、M2和K几种等能、等氮饲料,探讨其在海参养殖中的应用可行性。结果发现,抗菌肽添加组(K)刺参的体增重率(82.06%)显著高于对照组(C)(64.77%)(P<0.05),M1组比对照组提高13.4%,但差异不显著。免疫学指标测定中发现,刺参体腔细胞吞噬活性在各组间无显著差异,但各添加组体腔液超氧化物歧化酶(SOD),M2、K组溶菌酶(LSZ)和M1、M2组酸性磷酸酶(ACP)活性与对照组相比均表现出显著提高(P<0.05),其中SOD活性M1、M2组和K组分别比对照组提高了40.24%、49.91%和61.49%;LSZ活性M2组和K组分别比对照组提高6.41%和5.48%;ACP活性,M1组和M2组分别比对照组提高了29.65%和37.34%。经体腔注射用灿烂弧菌(Vibrio splendidus)侵染各组健康刺参,三处理组刺参均表现出一定的对病原菌的抵抗力,但其中K组刺参的免疫保护率最高。电镜切片显示,柞蚕抗菌肽和免疫蛹粉可以提高小肠绒毛的长度,但2.0%免疫蛹粉则对小肠形态产生一定程度的破坏。综上,饲料添加1.0%柞蚕免疫蛹粉可有效促进刺参生长,并提高抗病力。
Immunity induced pupae of Antheraea Pernyi powder（IPP）was produced by our institute,and supplemented into basic diets of sea cucumber with the different levels（0%,1.0% and 2.0%）,simultaneously 0.5% merchandise antimicrobial peptides was used as a refer.Four isonitrogenous and isoenergic diets contained different levels of IPP and antimicrobial peptides were formulated named group C,M1,M2 and K,respectively.After 60 days feeding trail,growth performance,immunity,disease-resistance and intestinal epithelial cells of sea cucumber with average weight of（1.24 ±0.41）g were investigated to discuss its feasible application in sea cucumber culture.Results showed that the weight gain rate of group K（82.06%）adding antimicrobial peptides was significantly higher than that of group C（64.77%）（P〈0.05）.Although group M1 increased by 13.4% than the control,no significant difference was found.As to the immunological parameters,no significant difference of phagocytic activity of coelomic cells was observed among groups,while activities of superoxide dismutase（SOD） of all treated groups,lysozyme（LSZ） of groups M2 and K,and acid phosphatase（ACP） of groups M1 and M2 were all significantly higher than that of the controls（P〈0.05）.Among them,the SOD of activity group M1,M2 and K increased by 40.24%,49.91% and 61.49%,respectively;the LSZ activity of group M2 and K increased by6.41% and 5.48%,respectively;while the ACP activity of group M1 ang M2 was found 29.65% and 37.34% higher than that of the control.Infectious disease test showed that sea cu cumber of three treated groups presented resistance against pathogenic bacteria to an certain extent,while group K showed the highest immune protection rate.An electron microscopic study was undertaken to investigate the effect of dietary IPP and antimicrobial peptides on intestinal villi and their villi promoting effect exhibited,however,2.0% IPP showed some damage to intestinal villi.From the above,1.0% IPP supplemented in the basic diets could increase growth performance and disease resistance of the sea cucumber in the study.
出处
《中国饲料》
北大核心
2016年第15期19-23,27,共6页
China Feed
基金
"十二五"国家科技支撑计划项目(2013BAD16B09-1)
现代农业产业技术体系建设专项(CARS-22)
辽宁省重大科技攻关项目(2014209001)
关键词
刺参
柞蚕免疫蛹粉
生长
免疫
肠绒毛
Apostichopus japonicus
immunity induced pupae of Antheraea Pernyi powder
growth
immunity
intestinal epithelial cells
