摘要
为提高五节芒可发酵单糖的产量,采用液氨对其进行预处理。选用预处理温度T、五节芒含水率M、驻留时间t和液氨用量比k为变量因素,在试验范围内T（70～170℃）、M（20%～200%）、t（5～30 min）、k（0.5～5.0）通过对各因素的不同取值,分析上述因素对五节芒酶解效果的影响。结果表明：在T=130℃、M=80%、t=10 min、k=2.0的条件下预处理,五节芒72 h的葡聚糖和木聚糖酶解率分别为72.1%和82.5%,即100 g干基原材料可获得可发酵单糖55.8 g,是未经预处理样品酶解率的3.65倍。因此,液氨预处理能够有效促进五节芒的酶解单糖转化率。
To effectively improve the enzymatic hydrolysis rate of carbohydrates in lignocelluloses,the liquid ammonia treatment（ LAT） was employed as pretreatment for Miscanthus floridulus. The pretreatment temperature T,Miscanthus floridulus moisture M,residence time t and the ratio of ammonia to biomass（ dry basis） k were chosen as variable factors. In the experimental ranges of T（ 70 ~170 ℃）,M（ 20% ~ 200%）,t（ 5 ~ 30 min） and k（ 0. 5 ~ 5. 0）,the influences of different variable factors on enzymatic hydrolysis of Miscanthus floridulus were investigated. The chemical composition analysis showed that the chemical constitution of Miscanthus floridulus was close to Saccharum arundinaceum and Miscanthus sinensis,and it could be a prior species of plant energy. The pretreatment methods and effects of these biomasses could be used as reference for each other. The parameter optimization results show that the optimal LAT pretreatment conditions for Miscanthus floridulus were130℃,80% moisture content,10 min residence time and 2. 0 ammonia to biomass ratio. Enzymatic hydrolysis using cocktail enzymes including cellulase, β-glucosidase and xylanase for 72 h after pretreatment under optimal conditions,the glucan and xylan hydrolysis rates of LAT-treated Miscanthus floridulus achieved 72. 1% and 82. 5%,which were 3. 3-fold and 4. 3-fold than those of untreated biomass respectively. The total fermentable monosaccharides after pretreatment under optimal conditions following enzymatic hydrolysis was 55. 8 g per 100 g of dry raw material. Compared with untreated substrates,it was about 3. 65-fold in sugar yield for LAT-treated substrates. As a result,the LAT process significantly reduced the recalcitrance of Miscanthus floridulus and facilitated biomass conversion to fermentable sugars.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2016年第7期196-201,共6页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家自然科学基金项目(31500491)
浙江农林大学生物质利用研究中心预研项目(2013SWZ03)
关键词
五节芒
预处理
液氨
酶解
Miscanthus floridulus
pretreatment
liquid ammonia
enzymatic hydrolysis
