摘要
目的:脑胶质瘤是发生率最高的恶性脑肿瘤,因具有高侵袭高浸润性的特点,使其预后效果差,中位生存期只有14.6个月,因此,寻找新的治疗药物或治疗手段意义重大。目前,许多研究发现漆黄素作为一种天然黄酮类化合物具有抗肿瘤作用,对多种肿瘤细胞具有明显抑制作用。然而漆黄素是否作用于脑胶质瘤细胞尚未得到深入探讨。方法:以U-87MG恶性胶质瘤细胞系作为研究对象,利用Transwell侵袭实验和Wound-healing划痕实验检测漆黄素处理后细胞的体外侵袭和迁移能力;利用MTS法检测细胞生长情况,利用流式细胞分析技术检测对漆黄素处理后细胞的细胞周期和细胞凋亡进行分析;利用Western-blot和Real-time PCR技术对相关基因的蛋白和mRNA表达水平进行检测。结果:漆黄素处理后,U-87MG细胞的侵袭力和迁移力显著降低,G2/M期细胞增多,细胞凋亡显著提高,同时相关基因的表达水平也有明显变化。结论:这些实验结果暗示漆黄素可通过调节细胞周期、促进细胞凋亡而明显降低脑胶质瘤细胞的侵袭和迁移力,从而对脑胶质瘤细胞具有显著的抗肿瘤作用。
OBJECTIVE Glioblastoma is the most frequent malignant form of brain cancer.For its high rate of invasion and migration,its median survival time is only 14.6 months.So it is essential to find out new therapeutic options.Fisetin,a naturally occurring flavonoid,has been shown to exert obviously anti-cancer effects against many types of tumor.However,whether fisetin can inhibit tumorigenesis of glioblastoma cells has not been fully researched.METHODS Effects of fisetin against invasion,migration,proliferation,cell cycle and apoptosis of U-87 MG treated with fisetin were detected via Transwell,Woundhealing,MTS,and flow cytometric analysis,respectively.Protein levels and mRNA expression levels of related genes were detected by Western-blot and Real-time-PCR assays,respectively.RESULTS Invasion and migration of U-87 MG were obviously inhibited,cell cycle progression was dramatically blocked in G2/M phase,and apoptosis also significantly increased.CONCLUSION These results strongly suggest that fisetin may exert its anti-tumor effects to glioblastoma by suppressing invasion and migration via inducing cell cycle arrest in G2/M phase and inducing cell apoptosis.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2016年第13期1051-1055,共5页
Chinese Journal of Hospital Pharmacy
基金
天津市卫生局科技基金项目(编号:2012KZ033)
