摘要
该实验基于前期黑水缬草抗老年痴呆（AD）的作用,对其有效部位进行化学成分研究,采用75%乙醇加热回流的方法对黑水缬草药材进行提取,提取物经萃取和大孔吸附树脂柱色谱分离得到其抗AD的有效部位,采用硅胶、ODS柱色谱以及制备型HPLC等色谱法分离该有效部位并得到9个化合物（1-9）,结合质谱及核磁等波谱技术,分别鉴定为6-hydroxy-7-（hydroxymethyl）-4-methy-lenehexahydrocyclopenta[c]pyran-1（3H）-one（1）,suspensolide F（2）,马钱子苷（3）,α-莫诺苷（4）,β-莫诺苷（5）,patrinovalerosidate（6）,野花椒苷A（7）,（-）-angelicoidenol-2-O-β-D-glucopyranoside（8）和citroside A（9）,其中化合物6-9为首次从缬草属植物中分离得到,进一步对化合物1-9进行体外抗AD活性研究发现化合物2和6对PC12神经元细胞损伤具有显著的保护作用。
In this study,the chemical constituents from Valeriana amurensis AD-effective fraction were investigated based on the effect of Valeriana amurensis on Alzheimer＇s disease（ AD） in previous study. Valeriana amurensis was extracted with 75% ethanol and the obtained extract were extracted and subjected to AB-8 macroporous resin column to obtain the AD-effective fraction of Valeriana amurensis. 9 compounds（ 1-9） were isolated with silica gel,ODS column chromatography and preparative HPLC. The structures of these compounds were determined as 6-hydroxy-7-（ hydroxymethyl）-4-methylenehexahydrocyclopenta[c]-pyran-1（ 3H）-one（ 1）,suspensolide F（ 2）,loganin（ 3）,α-morroniside（ 4）,β-morronisid（ 5）,partinovalerosidate（ 6）,zansiumloside A（ 7）,（-）-angelicoidenol-2-O-β-D-glucopyranoside（ 8）,citroside A（ 9）. Compounds 6-9 were isolated from the valerian genus for the first time and further investigated the anti-AD effect of compounds 1-9 in vitro found that compound 2 and 6 protected the PC12 cells from injury significantly.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第9期1649-1653,共5页
China Journal of Chinese Materia Medica
基金
黑龙江省自然科学基金项目(H2015040)
国家国际科技合作项目(2010DFA32440)
关键词
黑水缬草
老年痴呆
活性成分
结构鉴定
Valeriana amurensis
Alzheimer＇s disease
active constituents
structure identification
