摘要
目的建立甘草地上部分脂溶性成分高效液相指纹图谱,采用DPPH自由基清除能力、还原力、羟基自由基清除能力评价甘草地上部分脂溶性总黄酮抗氧化活性。方法建立甘草地上部分脂溶性黄酮类成分高效液相指纹图谱,色谱条件：岛津Wonda Sil-C18分析柱（4.6 mm×250 mm,5μm）,乙腈-0.1%磷酸-水溶液梯度洗脱,体积流量1 m L/min,柱温35℃,检测波长190-400 nm。通过体外抗氧化活性法测定甘草地上部分脂溶性总黄酮的清除DPPH自由基能力、还原能力及清除羟基自由基能力。结果甘草地上部分脂溶性黄酮类成分有良好的清除DPPH自由基活性、清除羟基自由基能力及还原能力。结论建立的HPLC-DAD指纹图谱方法能较好的反应甘草地上部分脂溶性黄酮类成分,甘草地上部分脂溶性总黄酮有较强的抗氧化能力。
Objective To establish the HPLC fingerprint of fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch.,the DPPH radical scavenging,reducing antioxidant power assay,hydroxyl free radical scavenging capacity was used to evaluate antioxidant activity of fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch.. Method HPLC fingerprint of the aerial parts of the fat-soluble flavonoids of Glycyrrhiza uralensis Fisch. was established,HPLC fingerprint chromatographic conditions： DIKMA Wonda Sil-C18 analytical column（ 4. 6 × 250 mm,5μm）,acetonitrile- 0. 1% phosphoric acid-water gradient,the volumetric flow rate was 1m L / min,column temperature was 35℃,detection wavelength was 190- 400 nm. In vitro antioxidant activity was measured by DPPH radical scavenging method,reducing power and scavenging hydroxyl radical assay. Results Fat-soluble flavonoids in aerial parts of Glycyrrhiza ura-lensis Fisch. had a strong antioxidant capacity. Conclusion HPLC-DAD fingerprint method can show fatsoluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch.. It has good DPPH radical scavenging activity,scavenging hydroxyl free radical activity and reducing power.
出处
《环球中医药》
CAS
2016年第5期567-570,共4页
Global Traditional Chinese Medicine
基金
国家“重大新药创制”科技重大专项(2009ZX09308-002)
国家中医药管理局行业专项(201107009-03)
关键词
甘草地上部分
脂溶性总黄酮
HPLC-DAD
Aerial parts of Glycyrrhiza uralensis Fisch.
Total fat-soluble flavonoids
HPLC-DAD
