摘要
棉花是我国的重要经济作物之一,棉花产量和纤维品质是评价棉花品种的重要指标,鉴于产量与品质性状的负向连锁,传统育种同步改良棉花产量和品质较为困难,鉴定棉花高产优质基因并导入优良育种品系是通过分子育种定向改良棉花产量与纤维品质的重要手段。已有研究表明iaaM基因在棉花胚珠表皮的特异表达能显著增强棉花衣分,改善纤维品质。本研究以转iaaM基因的高衣分品系'THL'为外源iaaM基因的供体亲本,以综合性状优良的'华杂棉H318'母本'B0011'为回交亲本,通过4代回交获得了BC4F1回交株系。利用SSR分子标记检测了这些株系的基因组回复率,并结合田间农艺性状考察获得具有外源iaaM基因、高衣分和低马克隆值等优异纤维品质性状的'B0011'改良的株系。在此基础之上通过选株、自交和分子鉴定获得了4个BC4F2株系,以这4个株系作为亲本与'华杂棉H318'的另一亲本'4-5'杂交获得导入iaaM基因的'华杂棉H318'。通过分子检测和田间试验分别考察了导入iaaM基因的'B0011'以及'华杂棉H318',并对iaa M基因导入该杂交种后对生长、产量和纤维品质等农艺性状的影响进行了分析。
Cotton is one of the important economic crops in China. Agronomical traits, like yield and fiber quality, are important criterions to evaluate cotton varieties. Due to the negative correlation in genetics, it is difficult to improve synchronously yield and fiber quality in traditional breeding. Identification of genes contributed to high yield and quality and application in breeding lines through genetic engineering is one of important means in molecular breeding to improve yield and fiber quality in cotton. Previous study shown that iaaM gene specifically expressed in cotton ovule epidermal could significantly improve the cotton lint percentage and fiber quality.In this study, iaaM gene was introduced into ＇B0011＇, one parent of＇Huazamian H318＇, with good comprehensive properties as the backcross parent, through backcross with the ＇THL＇ line as donor parent of iaaM gene.A BC4F1 population was obtained after 4 times backcross and genome reversion rate of these lines were evaluatedwith SSR molecular markers throughout the cotton genome. Individuals with iaaM gene, high lint percentage and fine quality were selected and self-crossed to generate BC4F2 population. ＇Huazamian H318＇ with iaaM gene was generated through BC4F2 crossing with ＇4-5＇, another parent of ＇Huazamian H318＇. Molecular identification and field investigation were applied in＇B0011＇ with iaaM gene and corresponding ＇Huazamian H318＇. The functions of iaaM genein development, yield and fiber quality in the hybrid were evaluated and discussed.
出处
《石河子大学学报（自然科学版）》
CAS
2016年第2期133-140,共8页
Journal of Shihezi University(Natural Science)
基金
国家高技术研究发展计划项目(2011AA10A102)
转基因重大专项(2013ZX08005-003-06)
