摘要
目的：探讨妊娠期糖尿病（gestational diabetes mellitus， GDM）孕妇足月胎盘Toll样受体4（Toll like receptor 4，TLR4）表达分布变化情况。方法选取2014年11月至2015年6月于北京大学第一医院剖宫产分娩的单胎足月妊娠GDM孕妇33例（无其他产科并发症和合并症），对照组为同期足月妊娠正常孕妇36例。应用免疫组织化学法检测胎盘组织TLR4蛋白表达分布，行半定量分析，并采用t检验比较胎盘各细胞类型TLR4表达变化情况。结果（1） TLR4表达于足月妊娠胎盘绒毛滋养细胞、蜕膜细胞、血管内皮细胞及羊膜上皮细胞的细胞膜、细胞质或细胞核中，着色不均匀。（2）与对照组相比，GDM组孕妇胎盘绒毛滋养细胞、蜕膜细胞TLR4表达明显增强（分别为0.38±0.01与0.31±0.01，0.39±0.01与0.34±0.01，t值分别为5.218和4.525，P值均＜0.01）。GDM组TLR4平均光密度值变化以绒毛滋养细胞变化最为显著。（3）与对照组相比，GDM组孕妇足月妊娠胎盘绒毛血管内皮细胞和羊膜上皮细胞TLR4表达无差异（P值均＞0.05）。结论 GDM孕妇足月妊娠胎盘组织不同类型细胞TLR4的表达变化存在差异。
Objective To evaluate the expression and distribution of Toll-like receptor 4 （TLR4） in term placentas of women with gestational diabetes mellitus （GDM）. Methods Placental samples were collected from 36 normal full-term singleton pregnant women （control group） and 33 full-term singleton pregnant women with GDM who had elective cesarean section in Peking University First Hospital between November 2014 and June 2015. Immunohistochemical assay was used to detect the expression and distribution of TLR4 in placental tissues. T test was used to compare the expression of TLR4 in various cell types of placenta by semi-quantitative analysis. Results （1） TLR4 was expressed in the cell membrane, cytoplasm or nuclei of trophoblast, decidual cells, vascular endothelial cells and amniotic epithelial cells of term placentas. （2） Compared with the control group, the expression of TLR4 was significantly enhanced in trophoblast and decidual cells of GDM women （0.38±0.01 vs 0.31±0.01, 0.39±0.01 vs 0.34±0.01, t=5.218 and 4.525, all P〈0.01）. Moreover, the change of TLR4 average optical density was most significant in trophoblast. （3） There was no difference in the expression of TLR4 in vascular endothelial cells and amniotic epithelial cells in term placentas of GDM women compared with the control group （all P〉0.05）. Conclusions The expression of TLR4 is different in various cell types of GDM term placentas.
出处
《中华围产医学杂志》
CAS
CSCD
2016年第5期350-354,共5页
Chinese Journal of Perinatal Medicine
基金
国家科学自然基金-青年科学基金项目（81300498）
国家重大科学研究项目（2015CB943304）
关键词
糖尿病
妊娠
胎盘
To
l
l样受体4
滋养层
Diabetes, gestational
Placenta
Toll-like receptor 4
Trophoblasts
