摘要
目的:了解川芎内生放线菌群结构,筛选拮抗川芎根腐病原菌的放线菌资源。方法:采用组织块法、匀浆法、划线平板法等对川芎根茎中的放线菌进行分离和纯化,平板对峙法及抑菌圈法评价各菌株抑菌活性,显微观察法结合DNA测序进行菌种物种鉴别。结果:共获得83株川芎内生放线菌,分属13个类群;其中链霉菌属灰红紫类群中的深海链霉菌Streptomyces scopuliridis(KF600747.1)、灰锈赤链霉菌Streptomyces griseorubiginosus(AB706352.1)、团孢链霉菌Streptomyces agglomeratus(LC055413.1)能同时抑制4种川芎根腐病原菌。结论:内生放线菌群中的链霉菌属灰红紫类群是抑制川芎根腐病原菌的重要生防菌,在川芎药材生产中有着重要的应用前景。
Objective：To get an understanding of the microflora of endophytic actinomyeetes in Ligusticum chuanxiong,and to ob- tain the resource of antagonistic strains against Ligusticum chuanxiong root rot disease. Methods ： Actinomycetes in stern nodes and rhizo- mes of Ligusticum chuanxiong were isolated and purified by a series of means, namely tissue block method, homogenate technique and plat streaking method. Further, dual culture and inhibition zone method were employed to test the antagonistic activity of these strains. To identify strains with potential precisely, both microscopic observation and DNA sequencing were conducted. Results： 83 strains of Ligusticum chuanxiong endophytic actinomycetes were identified, which consisted of 13 species groups. Among all of them, species identified as Streptomyces scopuliridis （ KF600747. 1 ）, Streptomyces griseorubiginosus （ AB706352. 1 ） and Streptomyces agglomera- tus （LC055413. 1 ） showed antagonistic activities against four kinds of pathogenic fungi of Ligusticum chuanxiong root rot disease. Conclusion ： Actinomycetes which belong to Griseorubroviolaceus groups of streptomyce are potential biocontrol microbes to Ligu- sticum chuanxiong root rot, showing importance to the production of Chuanxiong Rhizoma.
出处
《中药材》
CAS
CSCD
北大核心
2016年第2期265-269,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81001610)
四川省教育厅基金(14ZB0090)
高等学校博士学科点专项科研基金(20135132110004)
关键词
川芎
内生放线菌
根腐病
抑菌活性
Ligusticum chuanxiong Hort.
Endophytic actinomycetes
Root rot disease
Antagonistic activity
