摘要
目的建立应用十六烷基三甲基溴化铵(hexadecyltrimethyl ammonium bromide, CTAB)PCR产物纯化的方法。方法应用CTAB对PCR产物进行选择性沉淀。CTAB与DNA形成复合我1.2 mol/L NaCl溶液中溶解,再用乙醇将PCR产物沉淀出来。结果这种方法可以去除PCR产物中引物和小分子dNTP。虽然纯化的产率占现在市场上的一些试剂盒产率的80%,但其成本比较低,仍不失是一种PCR产物纯化的方法。结论利用十六烷基三甲基溴化铵可以用于PCR产物纯化。
Objective To establish a method for purifying PCR products with cetyltrimethylammonium bromide (CTAB). Method Selective precipitation of the PCR product was performed using CTAB, which forms compound with DNA fragment in salt solution of appropriate concentration, but not with single strain oligonucleotide or dNTPs. The precipitation could be dissolved in 1.2 mol/L NaCl while the addition of ethanol caused the desired PCR product to precipitate so as to be recovered. Result The primers and small-molecule dNTP could be effectively eliminated after this procedure. Although the output of the purification process reached only 80% that by current reagent kit, it reduces the cost to as low as 1/8 of that normally required by the kit. Conclusion CTAB is applicable for purification of the PCR product.
出处
《第一军医大学学报》
CAS
CSCD
北大核心
2002年第8期719-720,共2页
Journal of First Military Medical University
基金
国家自然科学基金(39880032)
关键词
十六烷基三甲基溴化铵
PCR产物
纯化
cetyltrimethylammonium bromide
polymerase chain reaction products
purification
