摘要
背景:利用骨髓间充质干细胞移植治疗缺血性脑血管病是目前的研究热点,移植后大多采用CT脑灌注扫描的方式了解缺血区血流改善情况,但CT脑灌注X射线照射可能会影响所移植骨髓间充质干细胞的活性,并影响到治疗效果。目的:探讨大鼠CT脑灌注扫描剂量对骨髓间充质干细胞活性的影响。方法:选取第5代SD大鼠骨髓间充质干细胞,随机分为3组,每组6管。从每组6管中随机选择1管不进行照射,其余5管分别进行1-5次CT脑灌注扫描。扫描结束之后,连续计数培养10 d的细胞数量,描绘各管细胞生长曲线,流式细胞仪检测各管细胞周期,MTT法检测细胞活性。结果与结论:连续计数10 d,各管细胞数量经比较差异均无显著性意义(P>0.05)。各管细胞的生长曲线基本重合。各管细胞的不同细胞周期比例和细胞活性差异均无显著性意义(P>0.05)。实验结果表明,常规进行CT脑灌注扫描对骨髓间质干细胞活性无影响。
BACKGROUND： Bone marrow mesenchymal stem cell transplantation is a hot spot in the treatment of ischemic cerebrovascular diseases, and CT brain perfusion scan is mostly used to observe theimprovement in the blood flow in the ischemic region. However, X-ray irradiation during CT brain perfusion may affect the viability of transplanted bone marrow mesenchymal stem cells, and then influence the therapeutic efficacy. OBJECTIVE： To investigate the effect of CT brain perfusion dose on the viability of bone marrow mesenchymal stem cells. METHODS： Passage 5 bone marrow mesenchymal stem cells from Sprague-Dawley rats were selected and randomized into three groups, with 6 tubes in each group. One of the six tubes in each group was randomly selected with no irradiation, and the remaining five tubes in each group were subjected to CT brain perfusion scans 1-5 times, respectively. After scanning, the cell number was counted after 10-day continuous culture and cell growth curve of each tube was drawn. Cell cycle was detected by flow cytometry, and cell viability was measured by MTT method. RESULTS AND CONCLUSION： After 10 days of continuous counting, the number of cells per tube showed no difference（P 〉0.05）, and cell growth curves were basically coincided. Moreover, there was no significant difference in the cell cycle and cell viability（P 〉0.05）. Experimental findings show that the CT brain perfusion scan has no effect on the viability of bone marrow mesenchymal stem cells.
出处
《中国组织工程研究》
CAS
北大核心
2016年第14期2027-2032,共6页
Chinese Journal of Tissue Engineering Research
