摘要
采用液液萃取法对山苍子乙醇粗提物进行分离和纯化,研究各萃取组分对赤拟谷盗的生物活性作用,并测定具有生物活性作用的萃取物的有效杀虫成分。结果表明:1.57 mg/cm2的氯仿萃取物对赤拟谷盗的驱避率达81.26%,50 mg/m L的氯仿萃取物对赤拟谷盗的触杀校正死亡率达75.98%。通过GC-MS检测出氯仿萃取物中含有机化合物共20种,其中羟基香茅醛相对含量为21.15%,2,6-二异丙基苯胺相对含量为16.41%,三氯叔丁醇相对含量为10.54%。对赤拟谷盗具有驱避、触杀作用的物质可能主要为羟基香茅醛和2,6-二异丙基苯胺。
The primary ethanol extracts of litsea cubeba were separated and purified by liquid- liquid extraction method. The biological activity of the extraction compositions against Tribolium castaneum was studied and the effective insecticidal compositions were determined. The results showed that the repellent rate of chloroform extracts at1. 57 mg / cm2 against Tribolium castaneum was 81. 26% and the contact-killing corrected mortality at 50 mg / m L was 75. 98%. The chloroform extracts contented 20 kinds of organic compounds by GC- MS and 7- hydroxy- 3,7- dimethyl- Octanal was 21. 15%,2,6- Diisopropylaniline was 16. 41%,Chlorobutanol was 10. 54%. The bioactivators possessed repelling and contact- killing may be 7- hydroxy- 3,7- dimethyl- Octanal,2,6- Diisopropylaniline.
出处
《粮油食品科技》
2016年第3期65-68,共4页
Science and Technology of Cereals,Oils and Foods
基金
海南省科学事业费项目(KYYS-2014-04)
关键词
山苍子
杀虫成分
分离
鉴定
litsea cubeba
insecticidal constituent
separation
identification
