摘要
利用农杆菌介导法将烟草铁蛋白基因Nt Fer1转入粳稻,经抗性筛选、PCR、Southern杂交、Northern杂交检测。结果表明,外源基因已整合到粳稻基因组中,并能稳定遗传表达。过量Fe2+营养液条件下培育自交T1代植株,研究表明转基因株系SOD和POD活性、叶绿素相对含量高于对照,而MDA含量则相反;转基因株系叶片和糙米总铁含量高于对照,所测16种氨基酸总含量与对照无显著差异,但部分氨基酸含量存在差异;接种稻瘟病混合小种菌液转基因株系稻瘟病叶瘟指数低于对照。烟草铁蛋白基因Nt Fer1转入表达可提高粳稻叶片和糙米储藏铁离子能力,增强对高铁胁迫耐性和稻瘟病叶瘟抗性。
We transformated tobacco ferritin gene Nt Fer1 into japonica genome by Agrobacterium mediated method, and the transformation was confirmed by resistance screening, PCR, Southern blotting and Northern blotting. T1 generation was cultured in overmuch Fe-（-（2＋））nutrient solution, and then we tested the physiological and biochemical indicators. The results showed that comparing to the wild type plants, SOD,POD activity and relative chlorophyll content of transgenic lines were increased, while the MDA content was decreased; the iron content in leaves and brown rice of transgenic lines were higher than the control, total amino acids（16 kinds） showed no significant difference, while some content had difference; after inoculation by rice blast mixed bacteria, the rice blast index of transgenic lines were lower than the control. Our research indicated that tobacco ferritin gene Nt Fer1 transgenic japonica could enhance the Fe^2＋storage ability of leaves and brown rice, and improve iron stress and rice blast resistance.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2016年第4期73-78,共6页
Journal of Northeast Agricultural University
基金
"十二五"农村领域国家科技计划课题(2013BAD20B04)
国家科技支撑计划项目(2011BAD16B11
2011BAD35B02-01-01)
