摘要
目的通过小干扰RNAs(siRNA)干扰泛素特异性蛋白酶7(USP7)在喉癌细胞中的表达,来探讨USP7对喉癌细胞生物学特性的影响。方法自行设计并使用高效siRNA在喉癌HEP2细胞株特异性干扰USP7表达,然后利用CCK-8法、Transwell小室迁移实验和流式细胞术观察USP7干扰后对喉癌细胞增殖、迁移能力和凋亡的影响。结果自行设计的siRNA可高效抑制喉癌HEP2细胞USP7mRNA及蛋白表达,显著抑制喉癌细胞的增殖、迁移能力,促进喉癌细胞的凋亡。结论 siRNAUSP7可以显著抑制喉癌细胞增殖和迁移能力,并促进凋亡,提示USP7可能是晚期喉癌治疗的一个重要靶标。
Objective This study is to investigate the influences of USP7 on the cytobiological characteristics of laryngeal cancer cells by small interfering RNA（siRNA）interfering the USP7 expression in the laryngeal cancer cells.Methods The self-designed highly efficient siRNA was used to conduct the specific interference on USP7 expression in laryngeal cancer HEP2 cells.Then the influence on the capacity of cell proliferation and migration,as well as apoptosise after USP7 interference were observed by using the CCK-8method,Transwell chamber migration test and flow cytometry.Results The self-designed siRNA could efficiently inhibit the expression of USP7 mRNA in laryngeal cancer cells,furthermore markedly suppressed the proliferation and migration of laryngeal cancer cells,enhanced the cell apoptosis in laryngeal cancer HEP2 cells in vitro.Conclusion The siRNA interfering USP7 can inhibit the proliferation and migration capacity of laryngeal cancer cells,and promoted their apoptosis.
出处
《重庆医学》
CAS
北大核心
2016年第12期1616-1619,共4页
Chongqing medicine
