摘要
目的从病理学角度评价啮齿类实验动物的健康情况,为实验动物质量评价提供依据。方法采集不同等级啮齿类实验动物的心脏、肝脏、脾脏、肺脏、肾脏、大肠和小肠,经10%中性福尔马林固定液固定,通过石蜡切片、HE染色、PAS染色、冰冻切片、油红O染色后于显微镜下观察。结果虽然被检测动物的微生物学、寄生虫学和遗传学等指标都能达到或符合国家质量标准,但是病理学监测中都能观察到不同程度的组织病理学损伤,主要出现在肝脏、肺脏和肾脏,有时也发生在心脏、消化道和淋巴器官等。结论该结果说明,虽然啮齿类实验动物符合国家微生物学等标准,但仍有部分实验动物处于亚健康状态。病理学监测在实验动物质量评价中有着不可替代的重要作用。相关监管部门应该尽快制定实验动物病理学诊断的国家及地方标准,完善实验动物质量监测体系。
Objective Assess the status of laboratory rodents by pathological diagnosis for offering some advices in standardized breeding to ensure accurate results of scientific research. Method Our laboratory collected the heart,liver,spleen,lung,kidney,large intestine and small intestine of animals,and put these tissues into 10%paraformaldehyde solution for fixation,and then prepared into two different sections for optical microscopy observation including all paraffin specimens stained with H. E. and the frozen sections stained with Oil Red-O and PAS. Result Although the vast majority of laboratory rodents were microbiological,parasitological and genetical diagnosed as healthy animals every years. There still exists different degree of pathological damage in them. Liver cell swelling,slight lung lesion and protein cast of kidney were main lesions which might affect the health of laboratory rodents. Sometimes we also observed various lesions in heart,digestive tract and lymphoid organs.Conclusions These results further demonstrate that though the health levels of laboratory rodents meet the microbiological national standards,there is still a part of them in sub-health status. Pathological monitoring plays an irreplaceable role in assessing the quality of laboratory rodents. Relevant regulatory departments should make the national and local pathological standardized policies for laboratory animals as soon as possible to improve the quality monitoring system of the experimental animals.
出处
《实验动物科学》
2016年第1期49-53,58,共6页
Laboratory Animal Science
基金
国家科技支撑计划
项目编号(NO.2015BAI07B02)
关键词
病理学监测
啮齿类实验动物
质量评价
pathological monitoring
laboratory rodents
quality assessment
