摘要
目的探讨不同检测方法在吸毒人群筛查丙肝中的应用价值。方法选取2014年1-8月中山市第二人民医院＂美沙酮门诊＂定点收治的吸毒史部分患者194例,采用HCV-c Ag ELISA试剂盒,HCV-Ab ELISA试剂盒,HCV-RNA PCR试剂盒分别检测。结果194例吸毒患者血样,HCV-RNA PCR阳性率为58.76%（114/194）,HCV-Ab阳性率为63.92%%（124/194）,HCV-c Ag阳性检出率为32.47%（63/194）。HCV-Ab标志物阳性率显著高于HCV-c Ag（χ^2=38.411,P〈0.01）。在HCV-Ab阴性样本中,有5例HCV-c Ag阳性,其中4例HCV-RNA PCR结果阳性。结论不同检测方法的检测效果略有差异,临床上可考虑HCV-c Ag与HCV-Ab联合检测优势互补,对吸毒高危人群联合筛查,将有效防止漏检。
Objective To explore the application value of different determination method in screening hepatitis C in drug users. Methods 194 patients with history of drug use sentinel treated by ＂adanon outpatient＂ in the Second People′s Hospital of Zhongshan City from January to August 2014 were selected.HCV-c Ag ELISA kit,HCV-Ab ELISA kit and HCV-RNA PCR kit was respectively used to detect. Results Among 194 samples from drug users,the positive rate of HCV-RNA PCR was 58.76%（114/194）,the positive rate of HCV-Ab was 63.92%（124/194）,the positive detection rate of HCV-c Ag was 32.47%（63/194）.The positive rate of HCV-Ab markers was obviously higher than t of HCV-c Ag（χ^2=38.411,P〈0.01）.Among HCV-Ab negetive samples,5 cases were HCV-c Ag positive and 4 cases with HCV-RNA PCR positive results among that. Conclusion The detection effect of different determination method has lightly difference,HCV-c Ag and HCV-Ab of combination detection may be considered for their complement each other′s advantages.Using combination screening to high-risk drug users can prevent missed detection effectively.
出处
《中国当代医药》
2016年第9期129-131,共3页
China Modern Medicine
基金
广东省中山市医学科研项目(2015A020183)
