摘要
将葡萄糖氧化酶（Glucose oxidase,GOD）固定在由多壁碳纳米管（MWCNTS）和石墨烯（Graphene）构成的纳米复合材料修饰的玻碳电极上,最外层用壳聚糖（Chitosan）进行覆盖,利用循环伏安法（Cyclic Voltammetry,CV）来测量葡萄糖氧化酶在修饰电极上的电化学和电催化反应,测得电子传递速率常数ks为0.87 s-1,电活性物质表面密度Г为1.54×10-10mol/cm-2,动力学表观米氏常数Km为1.32×103μmol/L,线性检测范围为：40-1000μmol/L,修饰电极有较好的稳定性,而且修饰电极在检测底物时有较好的抗干扰能力,因此,该体系修饰的电极有希望构建第三代葡萄糖生物传感器。
Direct electrochemistry of glucose oxidase was realized when it was immobilized on a nano-complex modified glassy carbon electrode,and the nano-complex was composed of graphene and multi-walled carbon nanotubes.The outermost layer covered with chitosan.Using cyclic voltammetry（CV） to measure electrochemical and electro-catalyzed reaction of glucose oxidase that modified electrode,the electron transfer rate constant（ks） was evaluated to be 0.87 s-1,and electroactive surface density was 1.54 × 10-10 mol / cm-2.The apparent Michaelis-Menten constant（Kappm） was 1.32 × 103μmol /L,linear detection range was 40 - 1000 μmol /L,and the modified electrode had better stability,and modified electrode had good anti-interference ability when detected the substrate.Therefore,the modified electrode can be used to detect glucose,as the third generation biosensors.
出处
《广州化工》
CAS
2016年第7期122-124,共3页
GuangZhou Chemical Industry
基金
河南大学特聘教授科研启动基金(No:5443D0810)
关键词
石墨烯
多壁碳纳米管
直接电化学
稳定性
抗干扰
graphene
multi-walled carbon nanotubes
direct electrochemistry
stability
anti-interference
