摘要
本次实验是应用单克隆斑点酶联免疫法(称Reverse-McAb-Dot-ELISA法)检测以布鲁氏菌感染小鼠后不同时期,其脏器中抗原存在情况,并同时分离细菌及进行某些血清学检查,比较其敏感性和特异性。结果表明,Reverse-McAb-Dot-ELISA具有较强的特异性,其不但可以提示受感染人群的保菌情况,并比细菌分离法敏感,比查抗体法(称正向)有特异、快速、安全、便于标本携带等优点,应作进一步探索。
The detection for Brucella antigens which existed in organs of mise infected with Brucella after 1,3, and 6 month with the Reverse-McAb-Dot-ELISA are reported. The bacterial incubation and serological tests were performed at same time so as to comoare their sensitivity and specificity. By means of the control, reproductive and coincidont tests,the results that the Reverse-McAb-Dot-ELISA were more sensitive than the bacterial incubation and more specifical than the present serological tests were indicated.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1991年第4期10-12,共3页
Chinese Journal of Zoonoses
关键词
布鲁氏菌病
抗原
酶联免疫法
Brucellosis Reverse-McAb,Dot-ELISA,Brucella antigen.
