摘要
旨在对克隆的牛ARRDC3和ARRDC4基因特性进行分析。以人ARRDC3和ARRDC4基因序列作探针,BLAST获得牛的表达序列标签(ESTs)序列,并设计引物利用RT-PCR方法从牛肩胛骨肌肉克隆c DNA序列,填充牛表达序列标签序列间的空缺,得到牛ARRDC3和ARRDC4基因。序列分析表明,牛ARRDC3基因编码区长1 245 bp,编码414个氨基酸;牛ARRDC4基因编码区长1 242 bp,编码413个氨基酸。氨基酸分析表明,ARRDC3蛋白主要分布在细胞质,不属于分泌蛋白;其磷酸化位点分布于丝氨酸(Ser)、苏氨酸(Thr)和酪氨酸(Tyr)残基上;二级结构预测以无规则卷曲为主;保守结构域预测含一Arrestin_C功能结构域。ARRDC4蛋白主要分布在细胞质和微体,不属于分泌蛋白;其磷酸化位点分布于丝氨酸(Ser)、苏氨酸(Thr)和酪氨酸(Tyr)残基上;二级结构以无规则卷曲为主;保守结构域预测含两段低复杂度序列和一Arrestin_C功能结构域。
In this study,the bovine ARRDC3 and ARRDC4 genes were cloned and the encoded proteins were analysed by bioinformatics methods. We chose human ARRDC3 and ARRDC4 genes to BLAST and got expressed sequence tags（ ESTs） of cattle. Sequence analysis showed that bovine ARRDC3 and ARRDC4 genes were amplified by sequencing assembling of ESTs of cattle and c DNA,which was amplified by RT-PCR from bovine scapula muscle. The bovine ARRDC3 contained a CDS region of 1 245 bp,encoded 414 amino acids; and the bovine ARRDC4 gene coding sequence was 1 242 bp,encoding 413 amino acids. The subcellular localization of ARRDC3 was in the cytoplasm and it did not belong to the secreted protein. The ARRDC3 protein contained a Arrestin_ C domain,and the phosphorylation site located in serine,threonine and tyrosine residue. The secondary structure of ARRDC3 was mainly composed of random coil. The subcellular localization of ARRDC4 was in the cytoplasm and microbody,and it did not belong to the secreted protein. The ARRDC4 protein contained a Arrestin_ C domain and two low complexity sequences,and the phosphorylation site located in serine,threonine and tyrosine residue. The secondary structure of ARRDC4 was mainly composed of random coil. The results above laid the foundation for further studies of expression regulation mechanism and function of ARRDC3 and ARRDC4 genes in the fat formation.
出处
《畜牧与兽医》
北大核心
2016年第4期39-45,共7页
Animal Husbandry & Veterinary Medicine
基金
高产转基因肉牛新品种培育(2014ZX08007-002)
