摘要
目的:研究远志寡糖酯对AD模型小鼠学习记忆障碍的改善作用及相关机制。方法:90只昆明种小鼠随机分为空白对照组,模型组,远志寡糖酯高、中、低剂量组(100、50、25 mg/kg)和多奈哌齐阳性对照组(0.75 mg/kg)。以腹腔注射氢溴酸东莨菪碱制备小鼠学习记忆获得障碍模型,通过跳台法及Morris水迷宫法观察小鼠的行为变化;并测定小鼠海马组织中乙酰胆碱酯酶(ACh E)活性、丙二醛(MDA)含量。结果:与空白对照组比较,小鼠注射氢溴酸东莨菪碱后出现明显的学习记忆障碍,海马组织中ACh E活性增加,MDA含量明显增加。与模型组比较,远志寡糖酯能明显减少模型小鼠的跳台错误次数,延长跳台潜伏期;能缩短小鼠在Morris水迷宫中的逃避潜伏期,增加撤台后穿台次数,缩短首次穿台时间;能降低海马组织中MDA含量,抑制ACh E活性。结论:远志寡糖酯能改善氢溴酸东莨菪碱所致小鼠学习记忆障碍,可能与其抗氧化、改善脑内胆碱能系统功能有关。
Objective：To study the effect of polygala oligosaccharide ester on scopolamine-induced learning and memory dysfunction in mice and its mechanism. Methods：90 KM mice were randomly divided into control group,model group,Polygala oligosaccharide esters group（100 mg/kg,50 mg/kg,25 mg/kg）and Donepezil group（0.75 mg/kg）. AD model mice were induced by scopolamine injection. The learning and memory were determined by the step-down test and Morris water maze. The acetyl cholinesterase（ACh E）activity and malondialdehyde（MDA）level in the hippocampus of mice were detected by biochemical kit. Results：Compared with vehicle control group,scopolamine could induce the learning and memory dysfunction in mice;increase the ACh E activity and the MDA level in hippocampus. Compared with AD model group,Polygala oligosaccharide esters（100 mg/kg,50 mg/kg,25 mg/kg）could attenuate the number of errors and prolong the step-down latency in the step-down test;shorten the escape latency on the fourth training day in Morris maze,shortened the latency of first-crossing the platform and increased the number of crossings of the platform within 90 s in the withdraw platform test and also decrease the ACh E activity and the MDA level in hippocampus. Conclusion：Polygala oligosaccharide ester has the improvement effect on learning and memory impairment mice induced by scopolamine hydrobromide,which maybe related with anti-oxidation and regulating cholinergic function.
出处
《辽宁中医药大学学报》
CAS
2016年第4期10-13,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81373995)
关键词
远志寡糖酯
东莨菪碱
学习记忆障碍
polygala oligosaccharide ester
scopolamine
learning and memory dysfunction
