摘要
目的优化复方石淋通胶囊的提取工艺,并与原提取工艺进行比较。方法运用正交试验,采用两种评价方法(评价方法Ⅰ:绿原酸、夏佛塔苷的含量及出膏率;评价方法Ⅱ:HPLC特征指纹峰的单位质量峰面积及出膏率)考察溶剂倍数、提取时间、醇沉浓度3个因素对新提取工艺的影响,并通过单因素试验考察提取次数对转移率的影响。应用层次分析法确定各指标权重系数,对实验结果进行综合评价。比较绿原酸和夏佛塔苷转移率,考察两种工艺的差异。结果两种评价方法筛选新工艺所得结果一致,优化后的复方石淋通胶囊最佳提取工艺为第1次加10倍量水,第2次加6倍量水,提取2次,每次1 h,浓缩液加入无水乙醇至醇沉浓度80%。新工艺的绿原酸和夏佛塔苷转移率明显高于原工艺。结论优化的提取工艺方法可行、工艺稳定、重复性好,保证了复方石淋通中各味药材中有效成分的提取,可适用于工业生产。
Objective To optimize the extraction techniques of compound Shi-Lin-Tong capsule and compare it with its original extraction technique. Methods The orthogonal test was used by two evaluating methods（ the first method was to determine the contents of chlorogenic acid and schaftoside and the extraction rate;the second method was to determine the HPLC characteristic fingerprint peak area and extraction rate） to investigate the influence of three factors on extraction factors（ solvent doses,extraction time and alcohol concentration; and the single factor test was used to investigate the effect of extraction frequency on the transfer rate of active ingredients. The analytic hierarchy technique was used to determine weight coefficients of each index and to evaluate the results of the test. The transfer rates of chlorogenic acid and schaftoside as well as the difference of two extraction techniques were compared. Results The outcomes obtained from the two evaluating methods were consistent. The optimized extraction technique of Shi-Lin-Tong capsule was as follows： the medicinal materials were extracted with ten times of water at the first extraction and six times of water at the second extraction,one hour for each time; and the concentrated solution was added to anhydrous ethanol until the ethanol concentration reaching 80% for ethanol precipitation. The transfer rates of chlorogenic acid and schaftoside of the optimized extraction technique was significantly higher than the original extraction technique. Conclusion The optimized extraction technique is feasible,stable,and repeatable,and facilitates to extract the effective components of compound Shi-Lin-Tong capsule and suits for industrial production.
出处
《上海中医药杂志》
2016年第4期88-93,共6页
Shanghai Journal of Traditional Chinese Medicine
基金
国家中信部科技成果转化资助项目(2060403)
关键词
复方石淋通
正交试验
层次分析法
多指标
指纹图谱
提取工艺
compound Shi-Lin-Tong capsule
orthogonal experiment
analytic hierarchy process
multiple index
fingerprint
extraction technique
