摘要
目的：探讨无创条件下建立食蟹猴的延迟性眨眼条件反射（ DEC）模型的方法并分析其行为习得规律。方法6只成年雄性食蟹猴，实验时利用特制猴椅进行清醒固定，其头部可自由活动，以500 ms、80 dB的纯音作为条件刺激（ CS），100 ms、5 psi的束状氧气流刺激角膜作为非条件刺激（ US），通过CS、US的反复配对训练其建立DEC，每个训练期120次，每日两个训练期。在此过程中，使用红外线反射探测法记录食蟹猴的眼睑活动，并对条件性眨眼反应（ CR）、惊吓反应（ SR）数据进行离线分析。结果6只食蟹猴中有4只完成了全部的DEC的训练过程，其中有3只动物成功习得DEC，CR率均值在最后一个训练期达到（64．67±2．00）％，（P＜0．01）；有1只动物仅在第1个训练期表现出较高的习得率（85．00％），而后每个训练期的CR率都很低，均值仅为18．16％，未能成功建立DEC模型。4只食蟹猴的SR发生率均较低，平均值为5．47％。结论虽然食蟹猴的DEC行为训练易于受到多种因素的影响，但在无创和适当制动的条件下，食蟹猴可以成功建立延迟性眨眼条件反射。
Objective To investigate the method of establishing a model of delay eyeblink condi-tioning （ DEC） in no surgical cynomolgus monkey and analyze the related acquisition rule.Methods Using the special monkey chairs to fix 6 adult male cynomolgus monkeys,they were sitting on the chairs and keep-ing awake,and heads could rotate freely during the whole training session.In the experiment,the 6 monkeys were trained in DEC with a tone （500 ms,85 dB） as the conditioned stimulus （CS）,paired with a corneal oxygen-puff （100 ms,5 psi） as the unconditioned stimulus （US）.There were 120 trials per-session,and 2 sessions per-day.During the experiments,an infrared emitter/detector attached to spectacles for eyeblink re-cording,the data of conditioned response （ CR） and startle response （ SR） were analyzed offline.Result-s Among the 6 cynomolgus monkeys,4 of them completed all of the training process of DEC and 3 monkeys had successful acquisition of DEC.The average CR rate in the last training session reached （ 64.67 ± 2.00）%,（P〈0.01）;1 monkey only showed a high acquisition rate（ 85.00%） at the first training session, and the low CR rate in the next training sessions with the average rate of 18.16%,suggesting that the DEC model was failed to established.The SR rates in 4 cynomolgus monkeys were low with average rate of 5.47%. Conclusion Although the DEC behavior training of the cynomolgus monkey is easy to be influenced by many inside or outside factors,DEC can be obtained under the condition of non surgery and proper braking.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2016年第3期198-203,共6页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目（81171249）
贵州省科学技术基金（黔科合J字[2015]2090号）
关键词
延迟性眨眼条件反射
食蟹猴
习得率
Delay eyeblink conditioning （DEC）
Cynomolgus monkey
Acquisition rate
