摘要
以双瓣茉莉花[Jasminum sambac(L.)Ait]花瓣为材料,采用RT-PCR和RACE技术相结合的方法,克隆了萜类合成酶基因(Js TPS)的全长cDNA,该cDNA全长为1 884 bp,其中ORF为1 491 bp,编码496个氨基酸的蛋白,分子量56 989.7 D,与原核表达结果一致。序列分析结果表明,该基因编码的氨基酸序列与油橄榄(Olea europaea)的TPS2具有75%的同源性,同属于α-Farnesene synthase分支。采用荧光定量PCR技术检测Js TPS在茉莉花开放过程中的表达量变化,结果表明,表达量在未开放时(18:00)最低,在半开放时(22:00)达到最高。
The full length cDNA of terpene synthase gene（Js TPS）was cloned by combination of RT-PCR and RACE from petals of Jasminum sambac. The results showed that full length cDNA contained 1 491 bp including an 1 884 bp ORF,encoding a 56 989.7 D protein with 496 amino acids whose molecular weight was consistent to that of product of the gene by prokaryotic expression;The result of alignment of amino acid showed that the gene had a homology of 75% to that of olive（Olea europaea）,belonging to α-Farnesene synthases;Quantities of the gene were detected by real-time PCR in process of opening of flower,the results showed that the expressing level of the gene was minimum at 18：00 when the flowers were not open,then had been increasing until at 22：00 when the expressing level reached to maximum and the flowers was opening.
出处
《园艺学报》
CAS
CSCD
北大核心
2016年第2期356-364,共9页
Acta Horticulturae Sinica
基金
福建省自然科学基金项目(2016J01110)
福州市科技局市校合作项目(2013-G-103)
福州市农业局2014年福州茉莉花茶产业提升项目(2014-3)
关键词
茉莉花
萜类合成酶
实时荧光定量PCR
原核表达
Jasminum sambac
terpene synthase gene
quantitative real-time PCR
prokaryotic expression
