摘要
目的:观察三甲散对大鼠肝星状细胞株HSC-T6Ⅰ、Ⅲ型胶原合成的影响。方法:以三甲散药物血清干预HSC-T6细胞,空白对照组采用DMEM培养液、HSC-T6,Y-27632阳性对照组采用Y-27632、DMEM培养液、HSC-T6,三甲散组采用三甲散、DMEM培养液、HSC-T6。ELISA法检测细胞上清Ⅰ型胶原和Ⅲ型胶原的含量,观察Ⅰ型胶原、Ⅲ型胶原合成情况。结果:与对照组比较,三甲散和Y-27632处理组I型胶原含量明显降低(P<0.01),三甲散和Y-27632处理组Ⅲ型胶原含量降低(P<0.05)。结论:三甲散可以抑制大鼠肝星状细胞株HSC-T6Ⅰ、Ⅲ型胶原合成。
Objective： To observe the influence of Sanjia Powder on the synthesis of HSC-T6 typeⅠand type Ⅲcollagen in rats.Methods： Intervene HSC-T6 cells with Sanjia Powder,blank control group with DMEM culture solution and HSC-T6,Y-27632 positive control group with Y-27632 and DMEM culture solution and intervene Sanjia Powder group with Sanjia Powder,DMEM culture solution and HSC-T6. The method of ELISA was applied to determine the content of typeⅠand type Ⅲcollagen in the cell supernatant and the synthesis of type Ⅰ and type Ⅲ collagen was observed. Results： Compared with control group,the content of type Ⅰ collagen in Sanjia Powder group and Y-27632 group declined obviously（ P 〈 0. 01）. The content of type Ⅲcollagen in Sanjia Powder group and Y-27632 group declined obviously（ P 〈 0. 05）. Conclusion： Sanjia Powder can inhibit the synthesis of HSC-T6 typeⅠand type Ⅲcollagen in rats.
出处
《河南中医》
2016年第3期428-430,共3页
Henan Traditional Chinese Medicine
基金
陕西省科技厅资助项目(编号:2013JQ4007)
