摘要
[目的]建立原代细胞培养大鼠耳蜗螺旋神经节神经元(SGN)的方法.[方法]选择出生后5d的SD大鼠的SGN进行原代细胞培养,采用免疫细胞染色法进行细胞学鉴定.[结果]新生SD大鼠SGN在原代培养条件下获得良好的细胞形态及表型分化,表现出稳定的神经元可塑性.[结论]原代细胞培养方法可获得状态良好的SGN细胞.
OBJECTIVETo create the primary culture of spiral ganglion neurons（SGN）of rats in vitro.METHODS The primary culture of SGN was carried out with five-day postnatal SD rats,and identification of cultured SGN was performed by immunocytochemistry.RESULTSThe dissociated SGN of SD rat could survive well and had a normal phenotypic differentiation in vitro to present the stable neuronal plasticity of SGN under the present experimental condition.CONCLUSIONThe SGN available in good condition can be cultured by the primary cell culture.
出处
《延边大学医学学报》
CAS
2015年第4期264-266,共3页
Journal of Medical Science Yanbian University
基金
国家自然科学基金项目
编号:81160127
