摘要
目的观察Th22细胞在卵巢癌恶性腹水(MA)中的分布情况,探讨其向腹腔内募集的可能机制。方法选择卵巢癌合并MA患者23例(观察组)、健康女性13例(对照组),用密度梯度离心法获得MA和外周血中单个核细胞,流式细胞仪检测MA和外周血中Th22、Th17细胞构成比及Th22细胞趋化因子表达水平,ELISA法检测腹水上清液和外周血中IL-17、IL-22的水平,用Spearman秩相关分析变量间关系。结果观察组MA中Th22、Th17细胞构成比高于同组外周血和对照组外周血(P均<0.05);观察组MA中CCR4、CCR7、CD45RO型Th22细胞构成比高于外周血,CD45RA、CD62L型低于外周血(P均<0.05);MA中IL-17、IL-22水平高于同组外周血及对照组外周血(P均<0.05);经Spearman秩相关分析,MA中Th22细胞与Th17细胞构成比呈正相关(r=0.66,P<0.01),IL-22水平与Th22细胞、Th17细胞构成比呈正相关(r分别为0.55、0.54,P均<0.05)。结论 Th22细胞可能通过趋化因子与炎症细胞因子由外周血向卵巢癌MA中募集。
Objective To observe the distribution of Th22 cells in malignant ascites（ MA） of ovarian cancer and to investigate its recruitment mechanism in the abdominal cavity. Methods Twenty-three cases of ovarian cancer patients with MA（ observation group） and 13 healthy women（ control group） were selected. Density gradient centrifugation was used to collect the mononuclear cells from MA and peripheral blood. The proportion of Th17 and Th22 cells from MA and peripheral blood,and the expression of chemokines in Th22 cells were detected by flow cytometry. The levels of IL-17 and IL-22 in MA and supernatant were examined by ELISA,and the correlations between Th22 cells and Th17 cells were determined by Spearman ＇s rank correlation coefficients. Results The proportion of Th22 and Th17 cells in MA was significantly higher than that in peripheral blood from the observation group and control group（ all P〈0. 05）. In the observation group,the proportions of Th22 cells in MA with CCR4,CCR7 and CD45 RO were higher and the proportions of Th22 cells in MA with CD45 RA and CD62 L were lower than those in the peripheral blood（ all P〈0. 05）. The levels of IL-17 and IL-22 in MA were higher than those from the peripheral blood in the observation group and control group（ all P〈0. 05）. Spearman＇s rank correlation coefficients showed that the proportion of Th22 cells was positively correlated with the proportion of Th17 cells in MA（ r = 0. 66,P 〈0. 01）,the level of IL-22 had a positive correlation with the proportion of Th22 cells and Th17 cells in MA（ r = 0. 55,0. 54; all P〈0. 05）. Conclusions Th22 cells may be recruited from peripheral blood to MA of ovarian cancer by chemokines and cytokines.
出处
《山东医药》
CAS
北大核心
2016年第9期7-9,共3页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81260083
31360221)
关键词
卵巢肿瘤
恶性腹水
辅助T细胞22
细胞分化
细胞募集
ovarian neoplasms
malignant ascites
helper T cells 22
cell differentiation
cell recruitment
