摘要
【目的】水生拉恩氏菌(Rahnella aquatilis)HX2能合成葡萄糖脱氢酶(glucose dehydrogenase,GDH)和吡咯喹啉醌(pyrroloquinoline quinone,PQQ),PQQ为GDH辅酶,与其共同参与葡萄糖溶磷代谢。揭示不同碳源条件下PQQ影响HX2菌株溶解无机磷作用的机理。【方法】以野生菌株HX2、突变体MH15及其互补菌株CMH15(pqq)为供试材料,在不同碳源条件下采用平板溶磷、钼锑抗比色法等方法对其进行溶解无机磷定性、定量以及p H相关分析。【结果】除D-山犁醇、D-果糖外,PQQ参与HX2菌株对木糖、葡萄糖、D-甘露糖、D-甘露醇、蔗糖、乳糖6种碳源的溶磷代谢,但其溶磷代谢因HX2菌株利用不同碳源的能力而不同,以乳糖利用最低,木糖利用最高。【结论】PQQ作为GDH的辅酶,两者共同参与HX2菌株的木糖、葡萄糖、D-甘露糖、D-甘露醇、蔗糖和乳糖溶解无机磷代谢,并起到重要调控作用。
【Objective】Rahnella aquatilis strain HX2 can produce glucose dehydrogenase（ GDH） and pyrroloquinoline quinone（ PQQ）. PQQ as coenzyme of GDH jointly take part in metabolism of phosphate solubilization under glucose source in HX2 strain. Based on prior researches,this paper further revealed effects of PQQ on inorganic phosphate solubilization under different carbon source conditions in HX2 strain. 【Method】HX2 wild strains,mutant MH15 and its complementary strains were used as test material. The methods of solubilzing phospate on plate,molybdenum- blue method and so on were used to analyze the qualitative and quantitative ability of phosphate solubilization under different carbon sources. 【Result】Except D- sorbitol and D- fructose,PQQ played a key role in HX2 phosphate solubilization with glucose,xylose,D- mannose,D- mannitol,sucrose and lactose as a sole carbon sources. However,its ability of phosphate solubilization was different with different carbon sources,which was the lowest for lactose and the highest for xylose as carbon sources. 【Conclusion】It has been clear that PQQ and GDH as holoenzyme were involved in metabolism of phospate solubilization under different carbon sources such as glucose,xylose,D- mannose,D- mannitol,sucrose and lactose,and played an important regulatory role in HX2 strain.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2016年第2期295-301,共7页
Xinjiang Agricultural Sciences
基金
自治区高校科研计划项目(XJEDU2014I041)
国家自然科学基金项目(31200386)~~
