摘要
目的观察节能灯、白炽灯和生物节律光源对抑郁模型大鼠行为学的影响,初步探讨其对褪黑素的影响机制。方法无特定病原体级健康雌性6周龄SD大鼠40只,适应性饲养后,随机取30只于11~14周龄时采用双侧卵巢切除联合孤养、慢性不可预知温和应激的复合式方法建立抑郁模型后,随机分为节能灯、白炽灯和生物节律灯干预组,每组10只,于17周龄时分别予相应的光源光照干预,为期3周;另10只大鼠为对照组,常规饲养。在17、19、20和21周龄时称量大鼠体质量,在7、14、20周龄时分别采用糖水偏好实验和旷场实验对大鼠进行行为学测试,在21周龄时测定大鼠1个暗周期（19：30）至明周期（08：30）7个时间点的周围血褪黑素水平,每组每个时间点随机测定1只大鼠。结果光照干预前的17周龄时,4组大鼠体质量差异无统计学意义（P〉0.05）;光照干预后,3个干预组大鼠体质量在17~20周龄均呈随时间增加而增长趋势（P〈0.05）;21周龄时4组大鼠体质量分别与同组20周龄比较,差异均无统计学意义（P〉0.05）。7周龄时,4组大鼠糖水消耗量和站立评分差异均无统计学意义（P〉0.05）;造模后未予光照干预前的14周龄时,3个干预组大鼠糖水消耗量和站立评分均低于对照组（P〈0.05）,3个干预组大鼠上述2个指标差异均无统计学意义（P〉0.05）;光照干预结束后20周龄时,4组大鼠糖水消耗量和站立评分差异均无统计学意义（P〉0.05）。3个干预组大鼠周围血褪黑素高峰值均高于对照组;节能灯和节律灯干预组大鼠周围血褪黑素高峰分别较对照组提前或延后2 h出现,而白炽灯干预组与对照组大鼠周围血褪黑素高峰出现时间相近。结论生物节律灯与节能灯、白炽灯对抑郁模型大鼠行为均有改善作用,3种光照干预效果相近;生物节律灯干预可延迟周围血褪黑素高峰出现时间。
Objective To observe the impact of energy saving light,incandescent light and circadian light on the ethology of depressive rats and explore its possible mechanism on affecting the secretion of melatonin. Methods Thirty rats aged 6weeks were randomly selected from 40 specific pathogen free health female SD rats after they adapted to the living environment,depressive rat models were established in the rats by bilateral ovariectomy combined with isolated living and chronic unpredictable mild stress stimulation at the age of 11-14 weeks. Then these 30 ovariectomized rats were randomly divided into 3 intervention groups,including an energy saving light group,an incandescent light group and a circadian light group,with 10 rats in each group. The rats in these 3 groups were given specific experimental light intervention for 3 weeks respectively at the age of 17 weeks. The other 10 rats were raised in conventional environment as the control group. Their body weights were measured at the age of 17,19,20 and 21 weeks. The ethology tests were carried out by sucrose preference test and the open-field test at the age of 7,14 and 20 weeks respectively. The melatonin levels in peripheral blood of 7 time points from 19： 30 to 8： 30 were measured in the rats at age of 21 weeks. One rat in each group at every time point was randomly selected for examination. Results At the age of 17 weeks before light-intervention,the body weights of rats in 4 groups showed no significant difference（ P〈0. 05）. After light-intervention,at the age of 17-20 weeks,the body weights of rats in 3 intervention groups were gradually increased with the increase of age（ P〈0. 05）.There was no significant difference between body weights of rats at the age of 21 weeks and those at the age of 20 weeks in each group（ P〈0. 05）. At age of 7 weeks,no significant differences were found in sucrose consumption and standing scores among these 4 groups（ P〈0. 05）. After the depressive models were established,at the age of 14 weeks before light-intervention,in rats of these 3 intervention groups,the sucrose consumption and standing scores were lower than those of the control group（ P〈0. 05）,and there was no significant difference found in the above 2 indexes among these 3intervention groups（ P〈0. 05）. At the age of 20 weeks after light-intervention,the sucrose consumption and standing scores were not significantly different from each other among the 4 groups（ P〈0. 05）. The peak levels of melatonin in the peripheral blood of rats in these 3 intervention groups were higher than that in the control group. The peak levels onsets of melatonin in peripheral blood of rats in the circadian light group and the energy saving light group were earlier or 2 hours delayed compared to that of control group,while it was similar between the incandescent light group and control group.Conclusion The circadian light,the energy saving light and the incandescent light are similarly effective in improving the behaviors of depressive rats. The circadian light can delay the onset of peak level of melatonin in peripheral blood.
出处
《中国职业医学》
CAS
北大核心
2016年第1期8-14,共7页
China Occupational Medicine
基金
国家高技术研究发展计划(863计划,2011AA03A113)
