摘要
目的:建立人乳腺癌MCF-7多西他赛(DTX)耐药细胞株,并对其耐药机制进行初步分析。方法:采用逐步提高多西他赛浓度、间歇诱导的方法,建立人乳腺癌MCF-7/DTX体外耐药细胞模型;耐药曲线检测MCF-7/DTX的耐药特性;流式细胞术比较耐药细胞株MCF-7/DTX及亲本细胞株MCF-7肿瘤干细胞含量的差异。结果:耐药曲线显示MCF-7/DTX比亲本细胞株MCF-7耐药;流式细胞分析显示MCF-7/DTX的细胞干细胞含量为2.59%,MCF-7细胞的干细胞含量为1.28%。结论:采用逐步提高浓度、间歇诱导的方法,建立了稳定、耐药性较高的MCF-7/DTX细胞株;干细胞含量升高是MCF-7/DTX的可能耐药机制之一。
Objective： To establish a human breast cancer cell line MCF-7 with the characterization of docetaxel （DTX） resistance, and investigate its biological mechanism of drug resistance. Methods： A DTX resistant human breast cancer cell line MCF-7/DTX was obtained discontinuously by gradually increasing doses of DTX. The drug re- sistance of MCF-7/DTX was evaluated by resistance curve. The effect of MCF-7/DTX on the content of breast can- cer stem cells was detected by flow cytometry. Results： Resistance curves showed that MCF-7/DTX was more re- sistant than MCF-7 cells. Flow cytometry showed that the content of MCF-7/DTX was 2.59%, and the content of MCF-7 ceils wad 1.28%. Conclusion： A drug-resistant cell line MCF-7/DTX was established by discontinuously in- duction and gradually increasing doses of DTX. The higher content of breast cancer stem cells is one of the mecha- nisms underlying the drug resistance of human breast cancer cell line MCF-7/DTX.
出处
《生物技术通讯》
CAS
2016年第1期63-65,共3页
Letters in Biotechnology
基金
国家自然科学基金(81372161)
关键词
多西他赛
乳腺癌
耐药
干细胞
docetaxel
breast cancer
drug resistance
stem cells
