摘要
为评价外源性雄激素17α-甲基睾酮(MT)对水生动物肝脏组织学结构的影响,对麦穗鱼雌鱼进行半静水MT暴露试验,探讨MT对麦穗鱼雌鱼的体重、肝脏重量、肝体指数以及肝脏组织学的影响。结果显示,MT暴露前期和中期,麦穗鱼体重变化不明显,但暴露后期体重显著降低(P<0.05);暴露于不同浓度的MT时,麦穗鱼雌鱼的肝脏重量与对照组相比均呈现不同程度的下降趋势;肝体指数的变化情况与肝脏重量一致,在暴露的中期和后期,麦穗鱼的肝体指数显著低于对照组(P<0.05)。不同浓度的MT暴露后,麦穗鱼肝脏均受到不同程度的损伤,出现肝脏空泡化、肝细胞核固缩等现象,且空泡化和核固缩现象随着MT浓度的升高和暴露时间的延长而加剧。
In order to understand the effect of exogenous androgen 17α-methyltestosterone （MT） on liver histological structure of aquatic animals, the semi-static water test was conducted using female Pesudorasbora parva as material to evaluate the effect of MT exposure on their body weight, liver weight, hepatosomatic index （HSI） and liver histology. The results showed that in early and middle phase of MT exposure, no significant changes in body weight of female Pesudorasbora parva were observed, however, in the late phase of MT exposure, their body weight were significantly reduced （P〈0.05）. The liver weight of female Pesudorcasbora parva which were subjected to exposure of different dosage MT were reduced in different degrees compared with that of CK. The change of HSI exhibited the similar trend with liver weight. In the middle and late phase of MT exposure, the HSI of female Pesudorasbora parva were significantly lower than that of CK （P〈0.05）. The histological injuries of liver after MT exposure were observed, including vacuolization and karyopyknosis of hepatocyte, and the injuries were gradually aggravated with the increase of MT dosage and extend of duration of MT exposure.
出处
《畜牧与饲料科学》
2016年第1期1-5,共5页
Animal Husbandry and Feed Science
基金
山西农业大学引进人才博士科研启动费项目(2014YJ08)
关键词
17α-甲基睾酮
麦穗鱼
肝脏
肝体指数
17ct-methyltestosterone
Pes udorasbora parva
liver
hepatosomatic index
